15-Deoxy-Δ-prostaglandin J2 induces chemokine expression, oxidative stress and microfilament reorganization in bovine mammary epithelial cells

Strandberg Lutzow, Ylva, Gray, Christian and Tellam, Ross (2008) 15-Deoxy-Δ-prostaglandin J2 induces chemokine expression, oxidative stress and microfilament reorganization in bovine mammary epithelial cells. Journal of dairy research, 75 1: 55-63. doi:10.1017/S0022029907003056


Author Strandberg Lutzow, Ylva
Gray, Christian
Tellam, Ross
Title 15-Deoxy-Δ-prostaglandin J2 induces chemokine expression, oxidative stress and microfilament reorganization in bovine mammary epithelial cells
Journal name Journal of dairy research   Check publisher's open access policy
ISSN 1469-7629
0022-0299
Publication date 2008-01-29
Sub-type Article (original research)
DOI 10.1017/S0022029907003056
Open Access Status Not Open Access
Volume 75
Issue 1
Start page 55
End page 63
Total pages 9
Place of publication Cambridge
Publisher Cambridge University Press
Language eng
Subject 06 Biological Sciences
Abstract The roles of the pro-adipogenic ligands of the transcription factor Peroxisome Proliferator Activated Receptor gamma (PPARG) in regulating innate immune responses in bovine mammary epithelial cells (bMEC) were investigated using quantitative real-time PCR. The analyses revealed that 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) enhanced the expression of Interleukin 8 (IL-8) and Chemokine (C-X-C motif) ligand 6 (CXCL6) in these cells in a dose-dependent manner. 15d-PGJ2 also induced the expression of transcripts encoding proteins involved in oxidative stress, including Ferritin heavy chain and Superoxide dismutase 1, as well as substantial microfilament reorganization. In contrast, synthetic PPARG agonists displayed a different and much smaller range of effects on the cells, causing down-regulation of Interleukin 1-beta, Interleukin 6 and IL-8 and increased expression of Chemokine (C-C motif) ligand 2 (CCL2) and Tumour necrosis factor alpha (TNFα). In an independent analysis, the cells were pre-incubated with PPARG agonists followed by lipopolysaccharide stimulation. This study revealed that troglitazone increased the responsiveness of the cells to lipopolysaccharide resulting in up-regulation of Interleukin 1-beta, TNFα, IL-8, CCL2 and CXCL6 while 15d-PGJ2 caused down-regulation of TNFα, CCL2 and CXCL6. These findings are relevant to understanding the anti-inflammatory potential of the PPARG ligands and underline different mechanisms of action of 15d-PGJ2 and troglitazone in bMEC. Furthermore, the present results demonstrate that the generation of pro-inflammatory mediators can be modulated by currently available therapeutic agents and may therefore be of value in the treatment of mastitis in ruminants.
Keyword Microfilament reorganization
15-deoxy-Δ12
14-prostaglandin J2
chemokine
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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Created: Mon, 30 Mar 2009, 22:01:55 EST by Ms Lynette Adams on behalf of School of Veterinary Science