MAP2 provides reliable early assessment of neural injury in the newborn piglet model of birth asphyxia

Lingwood, Barbara E., Healy, Genevieve N., Sullivan, Susan M., Pow, David V. and Colditz, Paul B. (2008) MAP2 provides reliable early assessment of neural injury in the newborn piglet model of birth asphyxia. Journal of Neuroscience Methods, 171 1: 140-146. doi:10.1016/j.jneumeth.2008.02.011

Author Lingwood, Barbara E.
Healy, Genevieve N.
Sullivan, Susan M.
Pow, David V.
Colditz, Paul B.
Title MAP2 provides reliable early assessment of neural injury in the newborn piglet model of birth asphyxia
Journal name Journal of Neuroscience Methods   Check publisher's open access policy
ISSN 1872-678X
Publication date 2008-06-15
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.jneumeth.2008.02.011
Open Access Status Not yet assessed
Volume 171
Issue 1
Start page 140
End page 146
Total pages 6
Editor Greg A. Gerhardt
Vincenzo Crunelli
Place of publication Amsterdam, Netherlands
Publisher Elsevier Science
Language eng
Subject C1
920111 Nervous System and Disorders
060105 Cell Neurochemistry
Abstract Reduction in microtubule-associated-protein-2 (MAP2) immunoreactivity is a sensitive and quantifiable early marker of neural injury in rats. This study assessed the reliability of MAP2 as an early marker of neural injury following hypoxia/ischaemia in neonatal piglets, and compared the effects of perfusion and immersion fixation on MAP2 immunoreactivity. Hypoxia was induced in newborn piglets (n = 23) by reducing the FiO2 to 4% for 0, 25, 35 or 50 min. Six hours after the end of hypoxia piglets were killed, and the brain removed and immunolabelled for MAP2. Significant reductions in MAP2 immunoreactivity were seen in cortex, hippocampus, basal ganglia and thalamus. Reductions correlated with duration of hypoxia, pH at the end of hypoxia, cerebral function monitor amplitude and cerebral impedance 6 h after hypoxia, and with early histological evidence of ischaemic changes. Regions with reduced immunoreactivity correlated with areas where damage is present in later histological examination in this model. Immersion fixation with postmortem delays up to 30 min did not affect MAP2 immunoreactivity compared to perfusion-fixed tissue. Results indicate that MAP2 immunoreactivity 6 h after hypoxia/ischaemia is a reliable marker of neural injury in the neonatal piglet.
Keyword cerebral hypoxia
Cerebral hypoxia–ischaemia
Microtubule-associated protein 2 (MAP2)
Brain injury
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
UQ Centre for Clinical Research Publications
School of Public Health Publications
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Citation counts: TR Web of Science Citation Count  Cited 17 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 29 Mar 2009, 23:42:20 EST by Amanda Barnett on behalf of UQ Centre for Clinical Research