Expression of FGF2 and TGFα and testis morphology during testicular hypertrophy subsequent to hemicastration in the neonatal boar

Wells, R., Scott, P. T., Harrison, D. K., Wreford, N. G., Duckett, R., Johnston, S. D. and D'Occhio, M. J. (2008) Expression of FGF2 and TGFα and testis morphology during testicular hypertrophy subsequent to hemicastration in the neonatal boar. Molecular Reproduction and Development, 75 6: 961-966. doi:10.1002/mrd.20743


Author Wells, R.
Scott, P. T.
Harrison, D. K.
Wreford, N. G.
Duckett, R.
Johnston, S. D.
D'Occhio, M. J.
Title Expression of FGF2 and TGFα and testis morphology during testicular hypertrophy subsequent to hemicastration in the neonatal boar
Formatted title
Expression of FGF2 and TGFα and testis morphology during testicular hypertrophy subsequent to hemicastration in the neonatal boar
Journal name Molecular Reproduction and Development   Check publisher's open access policy
ISSN 1098-2795
1040-452X
Publication date 2008-01-01
Sub-type Article (original research)
DOI 10.1002/mrd.20743
Volume 75
Issue 6
Start page 961
End page 966
Total pages 6
Editor Ralph B. Gwatkin
Place of publication New York, U.S.A.
Publisher Wiley-Liss
Language eng
Subject C1
070206 Animal Reproduction
830308 Pigs
0702 Animal Production
Formatted abstract
The objective was to ascertain fibroblast growth factor-2 (FGF2), epidermal growth factor (EGF), and transforming growth factor- (TGF) mRNA expression and testis morphology during accelerated testicular growth after hemicastration in the neonatal boar. On Day 10 after birth (Day 0), boars were assigned to control (n = 28), no treatment; hemicastrated (n = 28), left testis removed. The right testis in both groups (n = 7) was removed on Days 5, 10, 15, and 20. Expression of mRNA for FGF2, EGF, and TGF was determined by qRT-PCR using TaqMan®. Testicular morphology was determined on Day 15. On Day 10, hemicastrated boars had a greater (P = 0.01) testis weight (6.2 ± 0.8 g; mean ± SEM) than controls (4.3 ± 0.4 g) and on Day 15 testis weight in hemicastrated boars (8.8 ± 0.8 g) was twice (P < 0.01) that of control boars (4.2 ± 0.3 g). Seminiferous tubule volume was approximately doubled in hemicastrated boars (P < 0.01) and was associated with an increase (P < 0.01) in Sertoli cell number. Interstitial compartment volume was greater (P < 0.01) in hemicastrated boars. Leydig cell numbers were similar (P = 0.14) but volume was greater (P < 0.01) for hemicastrates. There were no differences (P > 0.05) between control and hemicastrated boars in TGF or FGF2 expression on Day 5 or Day 10, and EGF was not detected. It was concluded that upregulation of TGF or FGF2 expression is not a pre-requisite for enhanced testicular growth and increased Sertoli cell proliferation that occurs subsequent to hemicastration in the neonatal boar. Mol. Reprod. Dev. 75: 961-966, 2008. © 2008 Wiley-Liss, Inc.
Keyword Testis
Growth factors
Sertoli cells
Stereology
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Sat, 28 Mar 2009, 02:57:35 EST by Mrs Kathy Bachmann on behalf of School of Animal Studies