A simple in vitro model for investigating epithelial/mesenchymal interactions: keratinocyte inhibition of fibroblast proliferation and fibronectin synthesis

Harrison, D. A., Dalley, A. J. and MacNeill, S. (2005) A simple in vitro model for investigating epithelial/mesenchymal interactions: keratinocyte inhibition of fibroblast proliferation and fibronectin synthesis. Wound Repair and Regeneration, 13 6: 543-550. doi:10.1111/j.1524-475X.2005.00076.x


Author Harrison, D. A.
Dalley, A. J.
MacNeill, S.
Title A simple in vitro model for investigating epithelial/mesenchymal interactions: keratinocyte inhibition of fibroblast proliferation and fibronectin synthesis
Journal name Wound Repair and Regeneration   Check publisher's open access policy
ISSN 1067-1927
1524-475X
Publication date 2005-11-01
Year available 2005
Sub-type Article (original research)
DOI 10.1111/j.1524-475X.2005.00076.x
Open Access Status
Volume 13
Issue 6
Start page 543
End page 550
Total pages 8
Place of publication Malden, MA
Publisher Blackwell Science
Language eng
Subject 060106 Cellular Interactions (incl. Adhesion, Matrix, Cell Wall)
Abstract Hypertrophic scarring and graft contracture are major causes of morbidity after burn injuries. It is well established that application of a split-thickness skin graft reduces scarring and contraction, and cultured epithelial autografts have a similar effect. To investigate the influence of keratinocytes on fibroblast proliferation and fibronectin synthesis, we used an in vitro separated co-culture model in which epithelial sheets were cultured above fibroblast monolayers without physical contact. We also investigated the response of fibroblasts to keratinocyte- conditioned medium (KCM) obtained from confluent and subconfluent keratinocyte monolayers. Both cultured epithelial sheets, composed of adherent fully confluent keratinocytes, and their conditioned medium, reduced fibroblast proliferation. However, KCM from subconfluent keratinocytes stimulated fibroblast proliferation at low concentrations while inhibiting it at higher concentrations, indicating that keratinocytes can produce both mitogenic and growth-inhibiting factors for fibroblasts. KCM, but not epithelial sheet co-culture, also inhibited fibroblast fibronectin synthesis. This indicates regulation of fibroblast phenotype by soluble factors released by the keratinocyte and also suggests that there is a dialogue between keratinocytes and fibroblasts with respect to fibronectin production. We conclude that this separated co-culture model is a simple way to study epithelial/mesenchymal communication particularly with respect to the role of the fibroblast in wound healing. (WOUND REP REG 2005;13:543–550)
Keyword Cell Biology
Dermatology
Medicine, Research & Experimental
Surgery
Cell Biology
Dermatology
Research & Experimental Medicine
Surgery
CELL BIOLOGY
DERMATOLOGY
MEDICINE, RESEARCH & EXPERIMENTAL
SURGERY
Q-Index Code C1
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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