Antisense suppression of tau in cultured rat oligodendrocytes inhibits process formation

Gordon, David, Kidd, Grahame J. and Smith, Ross (2008) Antisense suppression of tau in cultured rat oligodendrocytes inhibits process formation. Journal of Neuroscience Research, 86 12: 2591-2601. doi:10.1002/jnr.21719


Author Gordon, David
Kidd, Grahame J.
Smith, Ross
Title Antisense suppression of tau in cultured rat oligodendrocytes inhibits process formation
Journal name Journal of Neuroscience Research   Check publisher's open access policy
ISSN 0360-4012
Publication date 2008-09-01
Sub-type Article (original research)
DOI 10.1002/jnr.21719
Open Access Status DOI
Volume 86
Issue 12
Start page 2591
End page 2601
Total pages 11
Editor Jean de Vellis
Place of publication Hoboken, N. J., United States
Publisher Wiley-Liss
Language eng
Subject C1
110106 Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)
970106 Expanding Knowledge in the Biological Sciences
Abstract The microtubule-associated protein tau is integral to neuronal process development and has a role in the pathogenesis of several neurodegenerative conditions. We examined possible roles for tau in cultured oligodendrocyte process formation by using antisense oligonucleotide treatment. Inhibition of tau synthesis with single oligonucleotides resulted in decreased tau protein levels and significantly shorter cellular processes. Simultaneous use of two nonoverlapping oligonucleotides caused a major reduction in tau levels and severely inhibited process outgrowth. The timing of oligonucleotide addition to oligodendrocyte cultures was important, with addition of antisense at the time of plating into culture having the most significant effect on morphology through reduction of tau expression. © 2008 Wiley-Liss, Inc.
Keyword Antisense
microtubules
oligodendrocytes
tau proteins
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes Published Online: 23 May 2008

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 27 Mar 2009, 21:39:43 EST by Glenda Chown on behalf of School of Chemistry & Molecular Biosciences