Protein delivery using nanoparticles based on microemulsions with different structure-types

Graf, A., Jack, K.S., Whittaker, A.K., Hook, S.M. and Rades, Y. (2008) Protein delivery using nanoparticles based on microemulsions with different structure-types. European Journal of Pharmaceutical Sciences, 33 4-5: 434-444. doi:10.1016/j.ejps.2008.01.013

Author Graf, A.
Jack, K.S.
Whittaker, A.K.
Hook, S.M.
Rades, Y.
Title Protein delivery using nanoparticles based on microemulsions with different structure-types
Journal name European Journal of Pharmaceutical Sciences   Check publisher's open access policy
ISSN 0928-0987
Publication date 2008-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.ejps.2008.01.013
Open Access Status
Volume 33
Issue 4-5
Start page 434
End page 444
Total pages 11
Editor Urtti, A.
Place of publication Netherlands
Publisher Elsevier
Language eng
Subject C1
030603 Colloid and Surface Chemistry
860899 Human Pharmaceutical Products not elsewhere classified
Abstract Poly(alkylcyanoacrylate) nanoparticles based on microemulsions with different structure-types and containing insulin as a model protein were prepared and characterised in this study. A phase diagram of the pseudoternary system isopropyl myristate, caprylocaproyl macrogolglycerides, polyglycerol oleate and water was established. All compounds used in this study were pharmaceutically acceptable and biocompatible. The area in the phase diagram containing optically isotropic, monophasic systems was designated as the microemulsion region. Systems within this region were identified as water-in-oil (w/o), bicontinuous and oil-in-water (o/w) microemulsions with viscosity, conductivity, differential scanning calorimetry and self-diffusion NMR. The size distributions of the resulting nanoparticles prepared by interfacial polymerisation from selected microemulsions using ethyl (2) cyanoacrylate and butyl (2) cyanoacrylate were unimodal but template- and monomer-dependent and ranged from 160 to 400nm. Entrapment and release of insulin were also studied. Entrapment ranged from 11.5 to 20.9% and a near zero-order release was observed after an initial burst. Release of insulin was monitored for 6h. Insulin-loaded nanoparticles were 320-350 nm in size. The microemulsion-structure was retained during the polymerisation process as determined by NMR. This study showed that these microemulsions with flexible formulation possibilities for the solubilisation of peptides and proteins depending on their microstructure could serve well as a platform for designing encapsulation processes for oral delivery of insulin. (C) 2008 Elsevier B.V. All rights reserved.
Keyword Pharmacology & Pharmacy
Pharmacology & Pharmacy
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: 2009 Higher Education Research Data Collection
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Citation counts: TR Web of Science Citation Count  Cited 28 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 27 Mar 2009, 00:56:13 EST by Lesley-Jayne Jerrard on behalf of Centre For Magnetic Resonance