Amphiphilic Triblock copolymers of Methoxy-poly(ethylene glycol)-b-poly(L-lactide)-b-poly(L-lysine) for enhancement of Osteoblast attachment and growth

Peng, Hui, Xiao, Yin, Mao, Xueli, Chen, Lan, Crawford, Ross and Whittaker, Andrew K. (2009) Amphiphilic Triblock copolymers of Methoxy-poly(ethylene glycol)-b-poly(L-lactide)-b-poly(L-lysine) for enhancement of Osteoblast attachment and growth. Biomacromolecules, 10 1: 95-104. doi:10.1021/bm800937g


Author Peng, Hui
Xiao, Yin
Mao, Xueli
Chen, Lan
Crawford, Ross
Whittaker, Andrew K.
Title Amphiphilic Triblock copolymers of Methoxy-poly(ethylene glycol)-b-poly(L-lactide)-b-poly(L-lysine) for enhancement of Osteoblast attachment and growth
Journal name Biomacromolecules   Check publisher's open access policy
ISSN 1525-7797
Publication date 2009-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1021/bm800937g
Open Access Status Not yet assessed
Volume 10
Issue 1
Start page 95
End page 104
Total pages 10
Editor A-C. Albertsson
Place of publication USA
Publisher American Chemical Society
Language eng
Subject C1
090301 Biomaterials
929999 Health not elsewhere classified
Abstract Amphiphilic triblock copolymers of methoxy-polyethylene glycol)-poly(L-lactide)-poly(L-lysine) (MPEG-b-PLLA-b-PLL) (M(n) = 8540-22 240) were synthesized through the ring-opening polymerization of N(epsilon)-(Z)-lysine-N-carboxyanhydrides (N(epsilon)-(Z)-Lys-NCA) using MPEG-b-PLLA-NH(2) as a macroinitiator. The triblock copolymers and diblock precursors were characterized by (1)H NMR, ATR-FTIR, and GPC. The chain lengths of each block could be controlled by varying the feed ratios of the monomers. The surface properties of films of PLLA modified by blending with the triblock copolymers were investigated by XPS and AFM and demonstrated an enrichment of PLL blocks on the surface of the PLLA film. No cytotoxicity was detected on a range of modified PLLA films arising from the incorporation of the triblock copolymers. The triblock copolymers MPEG-b-PLLA-b-PLL showed better surface properties in promoting osteoblast adhesion and proliferation compared with pure PLLA and PLLA modified with MPEG-b-PLLA diblock copolymers. This study demonstrated that the triblock copolymers containing free amino groups, which self-segregate on the surface of biodegradable polyesters, have potential for applications in cell delivery and tissue engineering.
Formatted abstract
Amphiphilic triblock copolymers of methoxy-poly(ethylene glycol)-poly(l-lactide)-poly(l-lysine) (MPEG-b-PLLA-b-PLL) (Mn = 8540−22240) were synthesized through the ring-opening polymerization of Nε-(Z)-lysine-N-carboxyanhydrides (Nε-(Z)-Lys-NCA) using MPEG-b-PLLA-NH2 as a macroinitiator. The triblock copolymers and diblock precursors were characterized by 1H NMR, ATR-FTIR, and GPC. The chain lengths of each block could be controlled by varying the feed ratios of the monomers. The surface properties of films of PLLA modified by blending with the triblock copolymers were investigated by XPS and AFM and demonstrated an enrichment of PLL blocks on the surface of the PLLA film. No cytotoxicity was detected on a range of modified PLLA films arising from the incorporation of the triblock copolymers. The triblock copolymers MPEG-b-PLLA-b-PLL showed better surface properties in promoting osteoblast adhesion and proliferation compared with pure PLLA and PLLA modified with MPEG-b-PLLA diblock copolymers. This study demonstrated that the triblock copolymers containing free amino groups, which self-segregate on the surface of biodegradable polyesters, have potential for applications in cell delivery and tissue engineering.
Keyword Biochemistry & Molecular Biology
Chemistry, Organic
Polymer Science
Biochemistry & Molecular Biology
Chemistry
Polymer Science
BIOCHEMISTRY & MOLECULAR BIOLOGY
CHEMISTRY, ORGANIC
POLYMER SCIENCE
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Thu, 26 Mar 2009, 23:07:36 EST by Lesley-Jayne Jerrard on behalf of Centre For Magnetic Resonance