The behavior of aged regenerated Bombyx mori silk fibroin solutions studied by 1H NMR and rheology

Zainuddin, Le, Tri T., Park, Yoosup, Chirila, Traian V., Halley, Peter J and Whittaker, Andrew K. (2008) The behavior of aged regenerated Bombyx mori silk fibroin solutions studied by 1H NMR and rheology. Biomaterials, 29 32: 4268-4274. doi:10.1016/j.biomaterials.2008.07.041


Author Zainuddin
Le, Tri T.
Park, Yoosup
Chirila, Traian V.
Halley, Peter J
Whittaker, Andrew K.
Title The behavior of aged regenerated Bombyx mori silk fibroin solutions studied by 1H NMR and rheology
Formatted title
The behavior of aged regenerated Bombyx mori silk fibroin solutions studied by 1H NMR and rheology
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
1878-5905
Publication date 2008-11-01
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2008.07.041
Open Access Status Not yet assessed
Volume 29
Issue 32
Start page 4268
End page 4274
Total pages 7
Place of publication Oxford, UK
Publisher Elseveir
Language eng
Subject 0904 Chemical Engineering
Abstract As part of a project to utilize the regenerated silk fibroin (RSF) membranes as a supporting matrix for the attachment and growth of corneal stem/progenitor cells in the development of tissue engineered constructs for the surgical restoration of the ocular surface, the behavior of the aged RSF solutions has been investigated. The solutions were produced from domesticated silkworm (Bombyx mori) cocoons according to a protocol involving successive dissolution steps, filtration and dialysis. The solutions were kept at 4 °C in a refrigerator for a certain period of time until near the gelation time. The changes in molecular conformation were studied by solution-state 1H NMR, while the flow of the solutions was characterized by rheological method. Upon ageing turbidity developed in solutions and the viscosity continuously decreased prior to a drastic increased near the gelation time. The 1H resonances of aged solutions showed a consistent downfield shift as compared to the 1H resonances of the fresh solution. Shear thinning with anomalous short recovery within a certain range of low shear rates occurred in both fresh and aged solutions. While the solutions behave as pseudo-plastic materials, the chain conformation in aged solutions adopted all secondary configurations with β-strand being predominant.
Keyword Silk fibroin
Rheology
1H NMR
Chain conformation
Pseudo-plastic flow
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Chemical Engineering Publications
 
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Created: Thu, 26 Mar 2009, 22:23:18 EST by Lesley-Jayne Jerrard on behalf of Faculty Of Engineering, Architecture & Info Tech