Laboratory studies on Australian isolates of Metarhizium anisopliae as a biopesticide for the cattle tick Boophilus microplus

Leemon, Diana Margaret and Jonsson, Nicholas N. (2008) Laboratory studies on Australian isolates of Metarhizium anisopliae as a biopesticide for the cattle tick Boophilus microplus. Journal of Invertebrate Pathology, 97 1: 40-49. doi:10.1016/j.jip.2007.07.006


Author Leemon, Diana Margaret
Jonsson, Nicholas N.
Title Laboratory studies on Australian isolates of Metarhizium anisopliae as a biopesticide for the cattle tick Boophilus microplus
Journal name Journal of Invertebrate Pathology   Check publisher's open access policy
ISSN 0022-2011
Publication date 2008-01-01
Sub-type Article (original research)
DOI 10.1016/j.jip.2007.07.006
Open Access Status Not yet assessed
Volume 97
Issue 1
Start page 40
End page 49
Total pages 10
Editor Federici, B.A.
Place of publication US
Publisher Academic Press
Language eng
Subject C1
070709 Veterinary Pathology
830301 Beef Cattle
Abstract Thirty-one isolates of Metarhizium anisopliae were bioassayed against the cattle tick (Boophilus microplus). More than half of the isolates showed a high degree of virulence to ticks. Radial growth curves for growth between 20 °C and 40 °C were obtained for all isolates. This information together with information on virulence will be important for the selection of isolates suitable to kill ticks on the surface of cattle. A biopesticide for cattle ticks must kill ticks rapidly at temperatures within the upper end of most isolates’ growth curves. It was also found that the time taken to achieve 100% tick mortality in vitro using a virulent isolate could be halved by applying conidia in a 10% oil emulsion. Scanning electron microscopy and light microscopy were used to investigate and compare the germination and penetration of conidia formulated in aqueous and oil formulations. It was found that conidia in both formulations were able to germinate and produce appressoria on the surface of ticks in less than 11 h. Marked weakness within 26 h, followed by extensive hyphal growth on the cuticle characterised the invasion of ticks by M. anisopliae.
Keyword Biological control
Metarhizium anisopliae
Boophilus microplus
Biopesticide
Tick control
Cattle
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Veterinary Science Publications
 
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Created: Thu, 26 Mar 2009, 21:52:33 EST by Narelle Poole on behalf of School of Veterinary Science