Could polyphosphate-accumulating organisms (PAOs) be glycogen-accumulating organisms (GAOs)?

Zhou, Yan, Maite, Pijuana, Zeng, Raymond J., Lu, Huabing and Zhiguo, Yuan (2008) Could polyphosphate-accumulating organisms (PAOs) be glycogen-accumulating organisms (GAOs)?. Water Research, 42 10-11: 2361-2368. doi:10.1016/j.watres.2008.01.003


Author Zhou, Yan
Maite, Pijuana
Zeng, Raymond J.
Lu, Huabing
Zhiguo, Yuan
Title Could polyphosphate-accumulating organisms (PAOs) be glycogen-accumulating organisms (GAOs)?
Journal name Water Research   Check publisher's open access policy
ISSN 0043-1354
1879-2448
Publication date 2008-05-01
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.watres.2008.01.003
Open Access Status Not yet assessed
Volume 42
Issue 10-11
Start page 2361
End page 2368
Total pages 8
Place of publication London, United Kingdom
Publisher I W A Publishing
Language eng
Subject C1
060504 Microbial Ecology
969999 Environment not elsewhere classified
Abstract Polyphosphate (poly-P) is known to be a key compound in the metabolism of polyphosphate-accumulating organisms (PAOs). In this study, a sludge highly enriched (80%) in Candidatus Accumulibacter phosphatis (hereafter referred to as Accumulibacter), a widely known PAO, was used to study the ability of these microorganisms to utilize acetate anaerobically under poly-P-limiting conditions. The biomass was subject to several anaerobic and aerobic cycles, during which the poly-P pool of PAOs was gradually emptied by supplying feed deficient in phosphate and washing the biomass at the end of each anaerobic period using media containing no phosphorus. After three cycles, phosphorus was hardly released but PAOs were still able to take up acetate and stored it as polyhydroxyalkanoates (PHA), as demonstrated by post-FISH chemical staining. Glycogen degradation increased substantially, suggesting PAOs were using glycogen as the main energy source. This is a key feature of glycogen-accumulating organisms (GAOs), which are known to compete with PAOs in enhanced biological phosphorus removal (EBPR) systems. The ratios between acetate uptake, polyhydroxybutyrate (PHB) and polyhydroxyvalerate (PHV) production, and glycogen consumption agree well with the anaerobic models previously proposed for GAOs.
Keyword Candidatus Accumulibacter phosphatis
Enhanced biological phosphorus removal (EBPR)
Polyphosphate-accumulating organism (PAO)
Glycogen-accumulating organism (GAO)
Polyphosphate
Glycogen
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
Advanced Water Management Centre Publications
 
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Created: Thu, 26 Mar 2009, 18:35:58 EST by Suzanne Read on behalf of Advanced Water Management Centre