Microbial diversity of mid-stage Palinurid phyllosoma from Great Barrier Reef waters

Payne, M. S., Hoj, L., Wietz, M., Hall, M. R., Sly, L. I. and Bourne, D. G. (2008) Microbial diversity of mid-stage Palinurid phyllosoma from Great Barrier Reef waters. Journal of Applied Microbiology, 105 2: 340-350. doi:10.1111/j.1365-2672.2008.03749.x

Author Payne, M. S.
Hoj, L.
Wietz, M.
Hall, M. R.
Sly, L. I.
Bourne, D. G.
Title Microbial diversity of mid-stage Palinurid phyllosoma from Great Barrier Reef waters
Journal name Journal of Applied Microbiology   Check publisher's open access policy
ISSN 1364-5072
Publication date 2008-08-01
Sub-type Article (original research)
DOI 10.1111/j.1365-2672.2008.03749.x
Open Access Status Not yet assessed
Volume 105
Issue 2
Start page 340
End page 350
Total pages 11
Place of publication Oxford, England
Publisher Wiley-Blackwell
Language eng
Subject C1
060501 Bacteriology
830106 Aquaculture Rock Lobster
Formatted abstract
Aims: This study aimed to determine the bacterial community associated with wild-caught, mid-stage larvae of spiny lobsters (Palinuridae) in their native oligotrophic marine environment, and to compare their diversity and composition with communities associated with aquaculture-reared larvae of the tropical rock lobster Panulirus ornatus.

Methods and Results: Bacterial clone libraries constructed from wild P. ornatus (two libraries) and Panulirus penicillatus (one library) larvae (phyllosoma) revealed a dominance of α-proteobacterial sequences, with Sulfitobacter spp.-affiliated sequences dominating both P. ornatus libraries and constituting a major portion of the P. penicillatus library. Vibrio-related sequences were rarely detected from wild phyllosoma clone libraries in contrast to similar studies of aquaculture-reared animals. Scanning electron microscopy analysis revealed low levels of bacterial colonization on the external carapace of wild phyllosoma, again in contrast to aquaculture-reared animals, which are often colonized with filamentous bacteria (mainly Thiothrix sp.) that compromise their health. Fluorescence in situ hybridization of sectioned wild phyllosoma tissue displayed low overall abundance of bacteria within the tissue and on external surfaces, with α-, β-, and γ-Proteobacteria being confirmed as members of this bacterial community.

Conclusions: The consistency in predominant clone sequences retrieved from the three libraries indicated a conserved microbiota associated with wild phyllosoma. In addition, the observed differences in the microbial composition and load of reared and wild phyllosoma are indicative of the different environments in which the animals live.

Significance and Impact of the Study: Bacterial disease during early larval stages is a major constraint currently hindering the development of an aquaculture industry for the ornate rock lobster P. ornatus. Knowledge of the microbial community associated with wild animals will be advantageous for the identification of bacteria that may promote animal health.
Keyword 16S rRNA
clone library
fluorescence in situ hybridization
Panulirus sp.
scanning electron microscopy
wild phyllosoma
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes Published Online: 20 Feb 2008

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 26 Mar 2009, 01:36:25 EST by Jennifer Falknau on behalf of School of Chemistry & Molecular Biosciences