TLR7 stimulation augments T effector-mediated rejection, of skin expressing neo-self antigen in keratinocytes

Zhong, Jie, Usriansyah Usriansyah, Rachel De Kluyver, Leggatt, Graham R., Fernando, Germain J. and Frazer, Ian H. (2008) TLR7 stimulation augments T effector-mediated rejection, of skin expressing neo-self antigen in keratinocytes. European Journal of Immunology, 38 1: 73-81. doi:10.1002/eji.200737599


Author Zhong, Jie
Usriansyah Usriansyah
Rachel De Kluyver
Leggatt, Graham R.
Fernando, Germain J.
Frazer, Ian H.
Title TLR7 stimulation augments T effector-mediated rejection, of skin expressing neo-self antigen in keratinocytes
Journal name European Journal of Immunology   Check publisher's open access policy
ISSN 0014-2980
Publication date 2008-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1002/eji.200737599
Open Access Status
Volume 38
Issue 1
Start page 73
End page 81
Total pages 9
Place of publication Weinheim, Germany
Publisher Wiley - V C H Verlag GmbH
Language eng
Subject C1
110709 Tumour Immunology
920102 Cancer and Related Disorders
Abstract Immunotherapy generally fails to induce tumour regression in spontaneously arising tumours. Failure is attributed to both tumour-related factors and an ineffective immune response. As a model of tumour immunotherapy, without the confounding effects of potential tumour-determined mechanisms of immune evasion, we studied the requirements for rejection of skin grafts expressing a neo-self antigen in somatic cells and not in antigen-presenting cells. When antigen expression was restricted to somatic cells, both CD4+ and CD8+ effector cells were required for graft rejection. Although freshly placed grafts were spontaneously rejected, healed grafts established under the cover of T cell depletion were not rejected even after T cell numbers recovered to a level where freshly placed grafts on the same animal were rejected, suggesting that healed skin grafts expressing a neo-self antigen only in somatic cells could not be rejected by primed recipients with functional effector T cells. Local TLR7 ligation induced inflammatory responses and rejection of healed grafts exposed to the TLR agonist but did not induce rejection of untreated healed grafts on the same animal. Thus, local pro-inflammatory signalling via TLR7 can promote effector T cell function against skin cells displaying their nominal antigen.
Keyword Inflammation
Keratinocytes
Neo-self antigen
T cell function
TLR
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
UQ Diamantina Institute Publications
 
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Created: Thu, 26 Mar 2009, 00:21:03 EST by Kylie Hengst on behalf of UQ Diamantina Institute