False-Negative results in nucleic acid amplification tests: do we need to routinely use two genetic targets in all assays to overcome problems caused by sequence variation?

Whiley, D. M., Lambert, S., Bialasiewicz, S., Goire, N., Nissen, M. D. and Sloots, T. P. (2008) False-Negative results in nucleic acid amplification tests: do we need to routinely use two genetic targets in all assays to overcome problems caused by sequence variation?. Critical Reviews in Microbiology, 34 2: 71-76. doi:10.1080/10408410801960913


Author Whiley, D. M.
Lambert, S.
Bialasiewicz, S.
Goire, N.
Nissen, M. D.
Sloots, T. P.
Title False-Negative results in nucleic acid amplification tests: do we need to routinely use two genetic targets in all assays to overcome problems caused by sequence variation?
Journal name Critical Reviews in Microbiology   Check publisher's open access policy
ISSN 1549-7828
1040-841X
Publication date 2008-05-01
Year available 2008
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1080/10408410801960913
Open Access Status
Volume 34
Issue 2
Start page 71
End page 76
Total pages 6
Editor M. Richardson
Place of publication Boca Raton, Fla.
Publisher CRC Press
Language eng
Subject C1
060502 Infectious Agents
060503 Microbial Genetics
060506 Virology
920109 Infectious Diseases
030405 Molecular Medicine
100402 Medical Biotechnology Diagnostics (incl. Biosensors)
Abstract Nucleic acid amplification tests (NAATs) have numerous advantages over traditional diagnostic techniques and so are now widely used by diagnostic laboratories for routine detection of infectious agents. However, there is some concern over the increasing numbers of reports of NAAT false-negative results caused by sequence variation. Highly conserved NAAT target sequences have been reported for many organisms, yet sequence-related problems continue to be observed in commercial and in-house assays targeting a broad range of microbial pathogens. In light of these ongoing problems, it may be time to consider the use of two genetic targets in NAAT methods to reduce the potential for sequence-related false-negative results.
Keyword NAAT
Nucleic Acid Fingerprinting
Amplification
Sequence
Variation
False-negative
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: 2009 Higher Education Research Data Collection
Clinical Medical Virology Centre Publications
 
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Created: Wed, 25 Mar 2009, 20:44:03 EST by Lesley Arnicar on behalf of Clinical Medical Virology Centre