DNA-based vaccines protect against zoonotic schistosomiasis in water buffalo

Da'Dara, A. A., Li, Y. S., Xiong, T., Zhou, J., Williams, Gail M., McManus, D. P., Feng, Z., Yu, X. L., Gray, Darren and Harn, D. A. (2008) DNA-based vaccines protect against zoonotic schistosomiasis in water buffalo. Vaccine, 26 29-30: 3617-3625. doi:10.1016/j.vaccine.2008.04.080

Author Da'Dara, A. A.
Li, Y. S.
Xiong, T.
Zhou, J.
Williams, Gail M.
McManus, D. P.
Feng, Z.
Yu, X. L.
Gray, Darren
Harn, D. A.
Title DNA-based vaccines protect against zoonotic schistosomiasis in water buffalo
Journal name Vaccine   Check publisher's open access policy
ISSN 0264-410X
Publication date 2008-05-19
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.vaccine.2008.04.080
Open Access Status DOI
Volume 26
Issue 29-30
Start page 3617
End page 3625
Total pages 9
Editor R. E. Spier
Place of publication United Kingdom
Publisher Elsevier
Language eng
Subject C1
920109 Infectious Diseases
110803 Medical Parasitology
11 Medical and Health Sciences
Abstract Schistosomiasis japonica is an endemic, zoonotic disease of major public health importance in China where water buffaloes account for approximately 75% of disease transmission. Interventions that reduce schistosome infection in water buffaloes will enhance their health simultaneously reducing disease transmission to humans. While chemotherapy has proved successful, it requires continued time consuming and expensive mass treatments. A more sustainable option would be development of vaccines that reduce transmission of S. japonicum from bovines to replace bovine chemotherapy. We performed two randomized double blind trials in water buffaloes to determine if DNA vaccines encoding triose-phosphate isomerase (SjCTPI), or the tetraspanin 23 kDa integral membrane protein (SjC23), alone or fused to bovine heat shock protein 70 (Hsp70) could induce a level of immunity conducive to long-term sustainable control. Groups of water buffaloes (15/group) received three intramuscular injections, 4 weeks apart. Booster immunizations were co-administered with a plasmid DNA encoding IL-12. Four weeks after the last injection, water buffaloes were challenged with 1000 cercariae, and vaccine efficacy analyzed 8 weeks later. Water buffaloes vaccinated with SjCTPI-Hsp70 or SjCTPI plasmids had worm burdens reduced by 51.2% and 41.5%, respectively. Importantly, fecal miracidial hatching was reduced by 52.1% and 33.2% respectively compared to control vaccinated water buffaloes. Vaccination with SjC23-Hsp70 and SjC23 plasmids reduced worm burdens by 50.9% and 45.5%, respectively, and fecal miracidial hatching by 52.0% and 47.4%. A mathematical model of schistosome transmission predicts that schistosome vaccines capable of reducing water buffaloes’ fecal egg output by 45%, alone or in conjunction with praziquantel treatment, will lead to a significant reduction in transmission of schistosomiasis. Both DNA vaccines tested here exceed this hypothetical level. Indeed, mathematical modeling of SjCTPI-Hsp70 and SjC23-Hsp70 alone and in conjunction with human chemotherapy showed a significant reduction in transmission almost to the point of elimination.
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Faculty of Health and Behavioural Sciences -- Publications
2009 Higher Education Research Data Collection
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 81 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 90 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 25 Mar 2009, 01:30:28 EST by Geraldine Fitzgerald on behalf of School of Public Health