Up-regulated expression of Sp1 protein coincident with a viral protein in human and mouse differentiating keratinocytes may act as a cell differentiation marker

Li, Bo, Wang, Xiao, Zhou, Fang, Saunders, Nicholas A., Frazer, Ian H. and Zhao, Kong-Nan (2008) Up-regulated expression of Sp1 protein coincident with a viral protein in human and mouse differentiating keratinocytes may act as a cell differentiation marker. Differentiation, 76 10: 1068-1080. doi:10.1111/j.1432-0436.2008.00300.x


Author Li, Bo
Wang, Xiao
Zhou, Fang
Saunders, Nicholas A.
Frazer, Ian H.
Zhao, Kong-Nan
Title Up-regulated expression of Sp1 protein coincident with a viral protein in human and mouse differentiating keratinocytes may act as a cell differentiation marker
Journal name Differentiation   Check publisher's open access policy
ISSN 0301-4681
Publication date 2008-12-01
Year available 2008
Sub-type Article (original research)
DOI 10.1111/j.1432-0436.2008.00300.x
Open Access Status
Volume 76
Issue 10
Start page 1068
End page 1080
Total pages 13
Place of publication Oxford, England
Publisher Blackwell
Language eng
Subject C1
920102 Cancer and Related Disorders
110709 Tumour Immunology
111201 Cancer Cell Biology
Abstract Sp1 is a transcription factor that regulates expression of mammalian and viral genes and is involved in different facets of cellular functions in eukaryotic cells. Here, we investigated Sp1 expression in primary mouse and human keratinocyte (KC) culture using quantitative reverse transcriptase polymerase chain reaction, Western blot, and immunofluorescence microscopy. Expression of Sp1 was post-transcriptionally up-regulated with increasing time in primary KC cultures. Sp1 expression, coincident with expression of human papillomavirus L1 capsid protein and involucrin, was associated with cell differentiation in vitro and in vivo in human and mouse skins. Immunoprecipitation experiments showed that Sp1 and L1 could be bound in a complex. Calcium (Ca(2+)) and all-trans retinoic acid are the positive modulators of KC differentiation, which positively and negatively regulated Sp1 and L1 expression. The data suggest that coincident expression of Sp1 with L1 proteins in differentiating KCs is mediated by a calcium-dependent signaling pathway.
Formatted abstract
Abstract Sp1 is a transcription factor that regulates expression of mammalian and viral genes and is involved in different facets of cellular functions in eukaryotic cells. Here, we investigated Sp1 expression in primary mouse and human keratinocyte (KC) culture using quantitative reverse transcriptase polymerase chain reaction, Western blot, and immunofluorescence microscopy. Expression of Sp1 was post-transcriptionally up-regulated with increasing time in primary KC cultures. Sp1 expression, coincident with expression of human papillomavirus L1 capsid protein and involucrin, was associated with cell differentiation in vitro and in vivo in human and mouse skins. Immunoprecipitation experiments showed that Sp1 and L1 could be bound in a complex. Calcium (Ca2+) and all-trans retinoic acid are the positive modulators of KC differentiation, which positively and negatively regulated Sp1 and L1 expression. The data suggest that coincident expression of Sp1 with L1 proteins in differentiating KCs is mediated by a calcium-dependent signaling pathway.
Keyword SP1
keratinocytes
cell differentiation
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 461023
301256
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
UQ Diamantina Institute Publications
 
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Created: Tue, 24 Mar 2009, 23:58:01 EST by Kylie Hengst on behalf of UQ Diamantina Institute