Lack of reproducible growth inhibition by Schlafen1 and Schlafen2 in vitro

Zhao, Liang, Neumann, Brent, Murphy, Kathleen, Silke, John and Gonda, Thomas J. (2008) Lack of reproducible growth inhibition by Schlafen1 and Schlafen2 in vitro. Blood Cells Molecules and Diseases, 41 2: 188-193. doi:10.1016/j.bcmd.2008.03.006

Author Zhao, Liang
Neumann, Brent
Murphy, Kathleen
Silke, John
Gonda, Thomas J.
Title Lack of reproducible growth inhibition by Schlafen1 and Schlafen2 in vitro
Journal name Blood Cells Molecules and Diseases   Check publisher's open access policy
ISSN 1079-9796
Publication date 2008-09-01
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.bcmd.2008.03.006
Open Access Status
Volume 41
Issue 2
Start page 188
End page 193
Total pages 6
Place of publication The United States of America
Publisher Academic Press
Language eng
Subject 970106 Expanding Knowledge in the Biological Sciences
920102 Cancer and Related Disorders
060103 Cell Development, Proliferation and Death
Abstract The Schlafen gene family has been implicated in lymphoid and myeloid maturation and differentiation as well as inflammation. However, little is known about the functions of this gene family except that anti-proliferative activities, particularly for Schlafen1, the prototype member of the family, have been reported. This was shown mainly by ectopic expression of Schlafen1 in murine fibroblasts resulting in growth inhibition and a G1 cell cycle arrest apparently via repression of Cyclin D1 expression. However, we have been unable to reproduce these findings. Schlafen1 and Schlafen2 failed to inhibit cell proliferation, cause G1 cell cycle arrest, or affect Cyclin D1 level in murine fibroblasts. This was regardless of whether overexpression was constitutive, induced or from transient transfections. Moreover, in our hands, Schlafen1 and -2 do not appear to regulate the activity of Cyclin D1 promoter. Importantly, we also showed that Schlafen1 and -2 do not play anti-proliferative roles in more physiologically-relevant myeloid cell lines. We therefore suggest that Schlafen1 and Schlafen2 might not have obligatory anti-proliferative activities, at least in vitro, and that efforts to explore their functions should be directed to other aspects, such as haemopoietic development and immune response.
Keyword Schlafen genes
Cyclin D1
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
UQ Diamantina Institute Publications
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Citation counts: TR Web of Science Citation Count  Cited 15 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 24 Mar 2009, 23:23:27 EST by Kylie Hengst on behalf of UQ Diamantina Institute