Strategies for the storage of Ancylostoma caninum third-stage larvae

Kopp, S. R., Kotze, A. C., McCarthy, J. S., Morton, J. M. and Coleman, G. T. (2008) Strategies for the storage of Ancylostoma caninum third-stage larvae. Journal of Parasitology, 94 3: 755-756. doi:10.1645/GE-1301.1


Author Kopp, S. R.
Kotze, A. C.
McCarthy, J. S.
Morton, J. M.
Coleman, G. T.
Title Strategies for the storage of Ancylostoma caninum third-stage larvae
Formatted title
Strategies for the Storage of Ancylostoma caninum Third-Stage Larvae
Journal name Journal of Parasitology   Check publisher's open access policy
ISSN 0022-3395
Publication date 2008-06-01
Year available 2008
Sub-type Article (original research)
DOI 10.1645/GE-1301.1
Open Access Status
Volume 94
Issue 3
Start page 755
End page 756
Total pages 2
Editor Esch, G.W.
Place of publication US
Publisher American Society of Parasitologists
Language eng
Subject 070708 Veterinary Parasitology
970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
C1
Abstract Although cryopreservation protocols for storage of hookworm larvae have been described, the circumstances under which the technique is necessary to ensure larval survival are not well defined. The motility of infective-stage larvae (as judged by observation) and their ability to migrate through canine skin in vitro were measured over a 7-mo period in worms held at room temperature and worms that had been cryopreserved at the start of the experiment. Cryopreserved worms showed motility and migration proportions of 45.6–48.0% and 26.8– 34.0%, respectively, throughout the experiment, compared with percentages of 92.7 and 84.1%, respectively, in the original fresh worms. Larvae held at room temperature showed a gradual decrease in motility and migration ability over the experimental period. Motility and migratory ability of cryopreserved larvae was only significantly higher (P < 0.01) than room temperature-stored larvae from 4 and 5 mo onward, respectively.
Keyword Hookworm Infection
cryopreservation
hookworm larvae
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Veterinary Science Publications
 
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Created: Tue, 24 Mar 2009, 22:46:29 EST by Narelle Poole on behalf of School of Veterinary Science