Mutation of the tumour suppressor p33ING1b is rare in melanoma

Stark, M., Puig-Butille, J. A., Walker, G., Badenas, C., Malvehy, J., Hayward , N. and Puig, S. (2006) Mutation of the tumour suppressor p33ING1b is rare in melanoma. British Journal of Dermatology, 155 1: 94-99. doi:10.1111/j.1365-2133.2006.07274.x


Author Stark, M.
Puig-Butille, J. A.
Walker, G.
Badenas, C.
Malvehy, J.
Hayward , N.
Puig, S.
Title Mutation of the tumour suppressor p33ING1b is rare in melanoma
Formatted title
Mutation of the tumour suppressor p33ING1b is rare in melanoma
Journal name British Journal of Dermatology   Check publisher's open access policy
ISSN 1365-2133
0007-0963
Publication date 2006-06-01
Sub-type Article (original research)
DOI 10.1111/j.1365-2133.2006.07274.x
Open Access Status
Volume 155
Issue 1
Start page 94
End page 99
Total pages 6
Place of publication London, U.K.
Publisher Blackwell
Language eng
Subject 111201 Cancer Cell Biology
111203 Cancer Genetics
Abstract Background The p33ING1b gene is involved in the p53-dependent response to DNA damage following exposure to ultraviolet radiation, and has recently been reported to be mutated in 20% of melanoma tumours. Objectives We sought to assess the p33ING1b mutation rate in our large panels of fresh melanomas and melanoma cell lines. Methods We screened 83 primary melanomas and 55 melanoma cell lines for mutations in p33ING1b by single-strand conformational polymorphism analysis and by direct sequencing. Results In contrast to previous reports, we found no somatic p33ING1b mutations in our panel of melanomas. We found that some of the discrepancy between our results and previously published studies may be due to inadvertent amplification of the ING1 pseudogene (INGX), and/or contamination of some samples with murine Ing1. Conclusions p33 ING1b mutations in melanoma are rare. We have highlighted the importance of allele-specific primer design to avoid pseudogene amplification, and also the necessity to confirm the genetic identity and species of origin of individual cell lines. Further studies are needed to clarify the possible role of p33ING1b in melanoma tumorigenesis.
Keyword ING1
Melanoma
Mutation
p33ING1b
Tumour suppressor
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Tue, 24 Mar 2009, 19:24:27 EST by Ms Lynette Adams on behalf of Medicine - Royal Brisbane and Women's Hospital