Flow cytometric detection of proteolysis in peptide libraries synthesised on optically encoded supports

Surawski, Peter P. T., Battersby, Bronwyn J., Lawrie, Gwendolyn A., Ford, Kym, Ruhmann, Andreas, Marcon, Lionel, Kozak, Darby and Trau, Matt (2008) Flow cytometric detection of proteolysis in peptide libraries synthesised on optically encoded supports. Molecular Biosystems, 4 7: 774-778. doi:10.1039/b718513h


Author Surawski, Peter P. T.
Battersby, Bronwyn J.
Lawrie, Gwendolyn A.
Ford, Kym
Ruhmann, Andreas
Marcon, Lionel
Kozak, Darby
Trau, Matt
Title Flow cytometric detection of proteolysis in peptide libraries synthesised on optically encoded supports
Journal name Molecular Biosystems   Check publisher's open access policy
ISSN 1742-206X
Publication date 2008-04-17
Sub-type Article (original research)
DOI 10.1039/b718513h
Open Access Status Not Open Access
Volume 4
Issue 7
Start page 774
End page 778
Total pages 5
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Language eng
Abstract The concept of optically encoding particles for solid phase organic synthesis has existed in the literature for several years. However, there remains a significant challenge to producing particles that are capable of withstanding harsh solvents and reagents whilst maintaining the integrity and range of the optical encoding. In this study, a new generation of fluorescently encoded support particles was used for both solid phase peptide synthesis and on-particle analysis of proteolysis in a multiplexed, flow cytometric assay. The success of the assay was demonstrated through the use of a model protease, trypsin. Our results show that the use of solid supports with high peptide yield, high swellability in water and high penetration of the enzyme into the interior of the particle is not absolutely necessary for proteolysis assays.
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Tue, 24 Mar 2009, 00:51:24 EST by Jennifer Falknau on behalf of School of Chemistry & Molecular Biosciences