Structure of SP-B/DPPC mixed films studied by neutron reflectometry

Fullagar, W.K., Holt, S.A. and Gentle, I.R. (2008) Structure of SP-B/DPPC mixed films studied by neutron reflectometry. Biophysical Journal, 95 10: 4829-4836. doi:10.1529/biophysj.108.134395


Author Fullagar, W.K.
Holt, S.A.
Gentle, I.R.
Title Structure of SP-B/DPPC mixed films studied by neutron reflectometry
Journal name Biophysical Journal   Check publisher's open access policy
ISSN 0006-3495
Publication date 2008-11-01
Year available 2008
Sub-type Article (original research)
DOI 10.1529/biophysj.108.134395
Open Access Status Not yet assessed
Volume 95
Issue 10
Start page 4829
End page 4836
Total pages 8
Place of publication United States
Publisher Biophysical Society
Language eng
Subject C1
030603 Colloid and Surface Chemistry
970103 Expanding Knowledge in the Chemical Sciences
Abstract The structures of films of pulmonary surfactant protein B (SP-B) and mixtures of SP-B and dipalmitoylphosphatidylcholine (DPPC) at the air/water interface have been studied by neutron reflectometry and Langmuir film balance methods. From the film balance studies, we observe that the isotherms of pure DPPC and SP-B/DPPC mixtures very nearly overlay one another at very high pressures, suggesting that the SP-B is being excluded from the film. The use of multiple contrasts with neutron reflectometry at a range of surface pressures has enabled the mixing and squeeze out of the DPPC and SP-B mixtures to be studied. We can identify the SP-B component of the interfacial structure and its position as a function of surface pressure. The mixtures are initially a homogeneous layer at low surface pressures. At higher surface pressures, the SP-B is squeezed out of the lipid layer into the subphase, with the first signs detected at 30 mN m(-1). At 50mN m(-1), the subphase is almost completely excluded from the DPPC layer, with the SP-B content significantly reduced. Only a small amount of DPPC appears to be associated with the squeezed out SP-B.
Keyword Biophysics
Biophysics
BIOPHYSICS
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Mon, 23 Mar 2009, 23:42:49 EST by Jennifer Falknau on behalf of School of Chemistry & Molecular Biosciences