The effects of acute exercise-induced cortisol on CCR2 expression on human monocytes

Okutsu, Mitsuharu, Suzuki, Katsuhiko, Ishijima, Toshimichi, Peake, Jonathan and Higuchi, Mitsuru (2008) The effects of acute exercise-induced cortisol on CCR2 expression on human monocytes. Brain, Behavior, and Immunity, 22 7: 1066-1071. doi:10.1016/j.bbi.2008.03.006

Author Okutsu, Mitsuharu
Suzuki, Katsuhiko
Ishijima, Toshimichi
Peake, Jonathan
Higuchi, Mitsuru
Title The effects of acute exercise-induced cortisol on CCR2 expression on human monocytes
Journal name Brain, Behavior, and Immunity   Check publisher's open access policy
ISSN 0889-1591
Publication date 2008-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.bbi.2008.03.006
Open Access Status DOI
Volume 22
Issue 7
Start page 1066
End page 1071
Total pages 6
Editor Kelley, K.W.
Place of publication San Diego, CA
Publisher Academic Press
Language eng
Subject C1
920108 Immune System and Allergy
950102 Organised Sports
110602 Exercise Physiology
11 Medical and Health Sciences
1106 Human Movement and Sports Science
Abstract CC-chemokine receptor 2 (CCR2) and its ligand, monocyte chemotactic protein-1 (MCP-1, also known as CCL2), are crucial for the recruitment of monocytes/macrophages to sites of inflammation. We conducted a series of experiments to investigate the relationship between stress, monocyte CCR2 expression and migration activity. First, we collected peripheral blood mononuclear cells (PBMC) from untrained subjects (n = 8) and measured CCR2 expression on CD14+ monocytes cultured with cortisol, epinephrine and norepinephrine. Second, we collected PBMC from the subjects before and after they cycled for 60 min at 70% peak O2 uptake (View the MathML source), and measured alterations in CCR2 expression on monocytes following exercise. Third, we cultured PBMC with serum obtained before and after exercise and the glucocorticoid antagonist RU-486 to determine the effect of cortisol on CCR2 expression in vitro. Last, we measured the ability of PBMC treated with serum or cortisol to migrate through membrane filters in response to CCL2. Cortisol (but not epinephrine or norepinephrine) increased CCR2 expression on monocytes in a dose- and time-dependent manner. Exercise did not influence CCR2 expression on PBMC, whereas incubation of PBMC with post-exercise serum significantly increased CCR2 expression. Both cortisol and post-exercise serum increased the migration of PBMC toward CCL2. The increase in CCR2 expression on PBMC following stimulation with cortisol and serum was blocked by the glucocorticoid receptor antagonist RU-486. In conclusion, cortisol released during exercise increased monocyte CCR2 expression and migration activity in vitro. These alterations may influence inflammation and regeneration of damaged tissue after acute stress.
Keyword Cell migration
Chemokine receptor
Exercise; Cortisol
Physical stress
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 18700563
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Human Movement and Nutrition Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 24 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 32 times in Scopus Article | Citations
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Created: Sun, 22 Mar 2009, 21:30:58 EST by Deborah Noon on behalf of Faculty Of Health Sciences