A Kunjin replicon vector encoding granulocyte macrophage colony-stimulating factor for intra-tumoral gene therapy

Hoang-Le, D., Smeenk, L., Anraku, I., Pijlman, G. P., Wang, X. J., De Vrij, J., Liu, W. J., Le, T. T., Schroder, W. A., Khromykh, Alexander A. and Suhrbier, A. (2009) A Kunjin replicon vector encoding granulocyte macrophage colony-stimulating factor for intra-tumoral gene therapy. Gene Therapy, 16 2: 190-199. doi:10.1038/gt.2008.169


Author Hoang-Le, D.
Smeenk, L.
Anraku, I.
Pijlman, G. P.
Wang, X. J.
De Vrij, J.
Liu, W. J.
Le, T. T.
Schroder, W. A.
Khromykh, Alexander A.
Suhrbier, A.
Title A Kunjin replicon vector encoding granulocyte macrophage colony-stimulating factor for intra-tumoral gene therapy
Journal name Gene Therapy   Check publisher's open access policy
ISSN 0969-7128
Publication date 2009-02-01
Year available 2008
Sub-type Article (original research)
DOI 10.1038/gt.2008.169
Open Access Status
Volume 16
Issue 2
Start page 190
End page 199
Total pages 10
Place of publication United Kingdom
Publisher Nature Publishing Group
Language eng
Subject C1
111204 Cancer Therapy (excl. Chemotherapy and Radiation Therapy)
920102 Cancer and Related Disorders
920407 Health Protection and/or Disaster Response
920412 Preventive Medicine
970111 Expanding Knowledge in the Medical and Health Sciences
Abstract We have recently developed a non-cytopathic RNA replicon-based viral vector system based on the flavivirus Kunjin. Here, we illustrate the utility of the Kunjin replicon system for gene therapy. Intra-tumoral injections of Kunjin replicon virus-like particles encoding granulocyte colony-stimulating factor were able to cure >50% of established subcutaneous CT26 colon carcinoma and B16-OVA melanomas. Regression of CT26 tumours correlated with the induction of anti-cancer CD8 T cells, and treatment of subcutaneous CT26 tumours also resulted in the regression of CT26 lung metastases. Only a few immune-based strategies are able to cure these aggressive tumours once they are of a reasonable size, illustrating the potential of this vector system for intra-tumoral gene therapy applications.
Keyword Kunjin replicon
granulocyte colony-stimulating factor
tumour
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Sat, 21 Mar 2009, 01:37:36 EST by Jennifer Falknau on behalf of School of Chemistry & Molecular Biosciences