A dual-purpose synthetic colloidal platform for protease mapping: substrate profiling for Dengue and West Nile virus proteases

Marcon, L., Kozak, D., Battersby, B.J., Chappell, K., Fairlie, D., Young, P.R. and Trau, M. (2008) A dual-purpose synthetic colloidal platform for protease mapping: substrate profiling for Dengue and West Nile virus proteases. Analytical Biochemistry, 2008 376: 151-153. doi:10.1016/j.ab.2008.01.034


Author Marcon, L.
Kozak, D.
Battersby, B.J.
Chappell, K.
Fairlie, D.
Young, P.R.
Trau, M.
Title A dual-purpose synthetic colloidal platform for protease mapping: substrate profiling for Dengue and West Nile virus proteases
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 0003-2697
Publication date 2008-02-02
Year available 2008
Sub-type Editorial
DOI 10.1016/j.ab.2008.01.034
Open Access Status
Volume 2008
Issue 376
Start page 151
End page 153
Total pages 3
Place of publication United States
Publisher Academic Press
Language eng
Subject C1
030302 Nanochemistry and Supramolecular Chemistry
060107 Enzymes
060506 Virology
970103 Expanding Knowledge in the Chemical Sciences
920109 Infectious Diseases
Abstract In a proof of concept study, we created a small focused fluorescent hexapeptide library onto 14 multiplexed barcoded sets of silica particles to probe the substrate recognition specificity of West Nile and Dengue virus proteases. A flow cytometric analysis demonstrated that the optical signature of each bead population remained distinguishable throughout the solid-phase peptide synthesis and proteolytic assay. As expected, both proteases displayed a narrow specificity for lysine and arginine residues in the P-1, and P-2 substrate positions. This open-ended platform enables the fast and simultaneous identification of peptide substrates and is applicable to other proteases. (c) 2008 Elsevier Inc. All rights reserved.
Keyword Biochemical Research Methods
Biochemistry & Molecular Biology
Chemistry, Analytical
Biochemistry & Molecular Biology
Chemistry
BIOCHEMICAL RESEARCH METHODS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CHEMISTRY, ANALYTICAL
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Editorial
Collections: 2009 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Sat, 21 Mar 2009, 00:32:55 EST by Jennifer Falknau on behalf of School of Chemistry & Molecular Biosciences