Regulatory interplay between pap operons in uropathogenic Escherichia coli

Totsika, M., Beatson, S.A., Holden, N. and Gally, D.L. (2008) Regulatory interplay between pap operons in uropathogenic Escherichia coli. Molecular Microbiology, 67 5: 996-1011. doi:10.1111/j.1365-2958.2007.06098.x


Author Totsika, M.
Beatson, S.A.
Holden, N.
Gally, D.L.
Title Regulatory interplay between pap operons in uropathogenic Escherichia coli
Formatted title
Regulatory interplay between pap operons in uropathogenic Escherichia coli
Journal name Molecular Microbiology   Check publisher's open access policy
ISSN 0950-382X
Publication date 2008-01-14
Year available 2008
Sub-type Article (original research)
DOI 10.1111/j.1365-2958.2007.06098.x
Open Access Status DOI
Volume 67
Issue 5
Start page 996
End page 1011
Total pages 16
Place of publication United Kingdom
Publisher Wiley-Blackwell
Language eng
Subject C1
060502 Infectious Agents
920109 Infectious Diseases
Abstract Pathogenic bacteria have a large repertoire of surface organelles involved in adherence, motility and protein export, but how individual bacteria co-ordinate surface organelle expression to prevent interference and excessive immune stimulation is unclear. Phase variation is a mechanism by which expression of surface factors is limited to a fraction of the bacterial population; however, the presence of multiple homologous surface structures controlled by related mechanisms and regulators antagonizes the independent expression achieved by phase variation. To investigate whether other mechanisms have evolved to sort out the bacterial cell surface, we examined regulatory cross-talk between multiple phase-variable pyelonephritis-associated pili (pap) operons in Escherichia coli isolates associated with urinary tract infections. Allelic variation identified in the regulatory regions and regulators acts synergistically to limit coexpression of homologous fimbrial operons. In particular, there is evidence that papI is under positive selection and PapI variants displayed differences in their capacity to activate related pap operons. Alleles of the high-affinity binding site for PapB were shown to contain a variable number of (T/A)(3) repeats occurring every 9 bp that altered the sensitivity of pap operon activation. Taken together with other examples of surface organelle cross-talk, we illustrate how this regulation could promote sequential expression.
Keyword Biochemistry & Molecular Biology
Microbiology
Biochemistry & Molecular Biology
Microbiology
BIOCHEMISTRY & MOLECULAR BIOLOGY
MICROBIOLOGY
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 069091/Z/02/Z
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 19 Mar 2009, 02:09:11 EST by Jennifer Falknau on behalf of School of Chemistry & Molecular Biosciences