In vivo visualization of albumin degradation in the proximal tubule

Slattery, Craig, Lee, Aven, Zhang, Yuan, Kelly, Darren J., Thorn, Peter, Nikolic-Paterson, David J., Tesch, Greg H. and Poronnik, Philip (2008) In vivo visualization of albumin degradation in the proximal tubule. Kidney International, 74 11: 1480-1486. doi:10.1038/ki.2008.463


Author Slattery, Craig
Lee, Aven
Zhang, Yuan
Kelly, Darren J.
Thorn, Peter
Nikolic-Paterson, David J.
Tesch, Greg H.
Poronnik, Philip
Title In vivo visualization of albumin degradation in the proximal tubule
Journal name Kidney International   Check publisher's open access policy
ISSN 0085-2538
1523-1755
Publication date 2008-12-01
Year available 2008
Sub-type Article (original research)
DOI 10.1038/ki.2008.463
Open Access Status
Volume 74
Issue 11
Start page 1480
End page 1486
Total pages 7
Place of publication Basingstoke, United Kingdom
Publisher Nature Publishing Group
Language eng
Formatted abstract
Albuminuria is a key marker of renal injury and a major risk factor for cardiovascular disease. In vivo imaging techniques with fluorescent albumin have allowed visualization of its movement within the whole kidney but they could not distinguish between intact and degraded albumin. To visualize albumin degradation in proximal tubular cells in vivo we used an albumin conjugate (dye quenched (DQ)-albumin), which only fluoresces when it is degraded. In cultured proximal tubule cells, the fluorescent signal from DQ-albumin was dependent on endocytosis and lysosomal function and showed that at any time about 40% of endocytosed DQ-albumin was degraded. Significant accumulation of conventional Texas Red-labeled albumin and degraded DQ-albumin was found in rat proximal tubules 5 min after injection. Importantly, no hint of DQ-albumin was detected in the serum, suggesting that the fluorescent signal in the proximal tubules was derived from tubular degradation of intact albumin. Our study shows that DQ-albumin, together with conventional fluorescent conjugates of intact albumin, can be used to visualize albumin degradation by proximal tubules in vivo.
Copyright 2008 International Society of Nephrology

Keyword Cells
Endocytosis
Microscopy, Confocal
Migration
Interacts
Kidney
CLC-5
Rat
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Wed, 18 Mar 2009, 22:04:00 EST