Epigenetic silencers are enriched in dormant desert frog muscle

Hudson, Nicholas J., Lonhienne, T. G. A,, Franklin, Craig E., Harper, Gregory S. and Lehnert, S. A. (2008) Epigenetic silencers are enriched in dormant desert frog muscle. Journal of Comparative Physiology B Biochemical, Systems, and Environmental Physiology, 178 6: 729-734. doi:10.1007/s00360-008-0261-0


Author Hudson, Nicholas J.
Lonhienne, T. G. A,
Franklin, Craig E.
Harper, Gregory S.
Lehnert, S. A.
Title Epigenetic silencers are enriched in dormant desert frog muscle
Journal name Journal of Comparative Physiology B Biochemical, Systems, and Environmental Physiology   Check publisher's open access policy
ISSN 0174-1578
1432-136X
Publication date 2008-08-01
Sub-type Article (original research)
DOI 10.1007/s00360-008-0261-0
Open Access Status Not yet assessed
Volume 178
Issue 6
Start page 729
End page 734
Total pages 6
Place of publication Germany
Publisher Springer
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
0606 Physiology
0608 Zoology
Formatted abstract
Green-striped burrowing frogs, Cyclorana alboguttata, survive droughts by entering a metabolic depression called aestivation, characterised by a reduction in resting oxygen consumption by 80%. Aestivation in C. alboguttata is manifest by transcriptional silencing of skeletal muscle bioenergetic genes, such as NADH ubiquinone oxidoreductase 1, ATP synthase and superoxide dismutase 2. In this study, we hypothesised that aestivation is associated with epigenetic change in frog muscle. We assessed mRNA transcript abundance of seven genes that code for proteins with established roles in epigenetically-mediated gene silencing [transcriptional co-repressor SIN3A, DNA (cytosine-5-) methyltransferase 1, methyl CpG binding protein 2, chromodomain helicase DNA binding protein 4, histone binding protein rbbp4, histone deacetylase 1 and nuclear receptor co-repressor 2] using qRT-PCR. These seven genes showed a modest (1.1–3.5-fold) but coordinated upregulation in 6-month aestivating muscle. This reached significance for SIN3A and DNA cytosine-5-methyltransferase 1 in standard pair-wise comparisons (p < 0.05), and the candidates as a whole when analysed by Fisher’s combined probability test (p < 0.01). These data are consistent with the hypothesis that the transcriptional silencing and metabolic depression that occurs during seasonal dormancy are associated with chromatin remodelling, and present a novel example of an environmentally induced epigenetic modification in an adult vertebrate.
Keyword Epigenetic
SIN3 co-repressor
Aestivation
Frog
Muscle
Dormancy
Disuse
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Biological Sciences Publications
Ecology Centre Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 19 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 03 Mar 2009, 03:30:05 EST by Gail Walter on behalf of School of Biological Sciences