Expression analysis of G Protein-coupled receptors in mouse macrophages

Lattin, Jane E., Schroder, Kate, Su, Andrew I., Walker, John R., Zhang, Jie, Wiltshire, Tim, Saijo, Kaoru, Glass, Christopher K., Hume, David A., Kellie, Stuart and Sweet, Matthew J. (2008) Expression analysis of G Protein-coupled receptors in mouse macrophages. Immunome Research, 4 5: . doi:10.1186/1745-7580-4-5

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Author Lattin, Jane E.
Schroder, Kate
Su, Andrew I.
Walker, John R.
Zhang, Jie
Wiltshire, Tim
Saijo, Kaoru
Glass, Christopher K.
Hume, David A.
Kellie, Stuart
Sweet, Matthew J.
Title Expression analysis of G Protein-coupled receptors in mouse macrophages
Journal name Immunome Research   Check publisher's open access policy
ISSN 1745-7580
Publication date 2008-04-29
Year available 2008
Sub-type Article (original research)
DOI 10.1186/1745-7580-4-5
Open Access Status DOI
Volume 4
Issue 5
Total pages 13
Place of publication Bedford Park, SA, Australia
Publisher The International Immunomics Society
Collection year 2009
Language eng
Formatted abstract
Background
Monocytes and macrophages express an extensive repertoire of G Protein-Coupled Receptors (GPCRs) that regulate inflammation and immunity. In this study we performed a systematic micro-array analysis of GPCR expression in primary mouse macrophages to identify family members that are either enriched in macrophages compared to a panel of other cell types, or are regulated by an inflammatory stimulus, the bacterial product lipopolysaccharide (LPS).
Results
Several members of the P2RY family had striking expression patterns in macrophages; P2ry6 mRNA was essentially expressed in a macrophage-specific fashion, whilst P2ry1 and P2ry5 mRNA levels were strongly down-regulated by LPS. Expression of several other GPCRs was either restricted to macrophages (e.g. Gpr84) or to both macrophages and neural tissues (e.g. P2ry12, Gpr85). The GPCR repertoire expressed by bone marrow-derived macrophages and thioglycollate-elicited peritoneal macrophages had some commonality, but there were also several GPCRs preferentially expressed by either cell population.
Conclusion
The constitutive or regulated expression in macrophages of several GPCRs identified in this study has not previously been described. Future studies on such GPCRs and their agonists are likely to provide important insights into macrophage biology, as well as novel inflammatory pathways that could be future targets for drug discovery.
Keyword Coupled receptors
GPCR
Mouse macrophages
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Doi broken, OA pdf added.

 
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Created: Tue, 24 Feb 2009, 19:51:06 EST by Cody Mudgway on behalf of Institute for Molecular Bioscience