Simultaneous quantification of differently glycosylated, acetylated, and 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one-conjugated soyasaponins using reversed-phase high-performance liquid chromatography with evaporative light scattering detection

Decroos, K., Vincken, J. P., Heng, L., Bakker, R., Gruppen, H. and Verstraete, W (2005) Simultaneous quantification of differently glycosylated, acetylated, and 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one-conjugated soyasaponins using reversed-phase high-performance liquid chromatography with evaporative light scattering detection. Journal of Chromatography A, 1072 2: 185-193. doi:10.1016/j.chroma.2005.03.021


Author Decroos, K.
Vincken, J. P.
Heng, L.
Bakker, R.
Gruppen, H.
Verstraete, W
Title Simultaneous quantification of differently glycosylated, acetylated, and 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one-conjugated soyasaponins using reversed-phase high-performance liquid chromatography with evaporative light scattering detection
Journal name Journal of Chromatography A   Check publisher's open access policy
ISSN 0021-9673
Publication date 2005-04-29
Sub-type Article (original research)
DOI 10.1016/j.chroma.2005.03.021
Open Access Status Not Open Access
Volume 1072
Issue 2
Start page 185
End page 193
Total pages 9
Place of publication Amsterdam
Publisher Elsevier
Language eng
Subject 100204 Environmental Biotechnology Diagnostics (incl. Biosensors)
Formatted abstract
A novel method utilizing high-performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) and electrospray ionisation mass spectrometry (ESI-MS) was developed for the analysis of soyasaponins, a divers group of triterpenic compounds with one or two sugar side chains, occurring in soy. Group A soyasaponins in different degrees of acetylation, as well as group B soyasaponins in both their 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP)-conjugated and non-conjugated forms could be separated and quantified using authentic soyasaponin standards, in one single run. The method was tested by the determination of the soyasaponin content and composition of eight soygerm samples of different origin. Differences in the composition and the degree of acetylation of the group A soyasaponins were observed among these samples. The group B soyasaponins showed much less variability and they were mainly present in their DDMP-conjugated form.
Keyword Soy
Soygerm
Soyasaponin
Reversed phase HPLC
Evaporative light scattering detection
DDHP
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 31 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 32 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 17 Feb 2009, 01:10:15 EST by Juliette Grosvenor on behalf of Library Corporate Services