Isolation of Amphimedon Developmental Material

Leys, Sally P., Larroux, Claire, Gauthier, Marie, Adamska, Maja, Fahey, Bryony, Richards, Gemma S., Degnan, Sandie M. and Degnan, Bernard M. (2008) Isolation of Amphimedon Developmental Material. Cold Spring Harbor Protocols, 3 12: . doi:10.1101/pdb.prot5095

Author Leys, Sally P.
Larroux, Claire
Gauthier, Marie
Adamska, Maja
Fahey, Bryony
Richards, Gemma S.
Degnan, Sandie M.
Degnan, Bernard M.
Title Isolation of Amphimedon Developmental Material
Formatted title
Isolation of Amphimedon Developmental Material
Journal name Cold Spring Harbor Protocols   Check publisher's open access policy
ISSN 1559-6095
Publication date 2008-12-01
Sub-type Article (original research)
DOI 10.1101/pdb.prot5095
Volume 3
Issue 12
Total pages 4
Place of publication United States
Publisher Cold Spring Harbor Laboratory Press
Language eng
Subject C1
960808 Marine Flora, Fauna and Biodiversity
060305 Evolution of Developmental Systems
Formatted abstract
Fertilization occurs internally in Amphimedon and embryos are brooded in multiple chambers throughout the adult. Each chamber contains a mixture of developmental stages, from egg to late ring stages (i.e., prehatch late embryos). At the end of embryogenesis, swimming parenchymella larvae emerge from the adult. After several hours in the water column, the larvae settle and metamorphose into juvenile sponges. This protocol details how to obtain Amphimedon larvae and post-larvae/juveniles as well as embryos. Once isolated, these biological stages can be used for a variety of molecular and cellular analyses.
Q-Index Code C1
Q-Index Status Confirmed Code

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Created: Sat, 14 Feb 2009, 03:11:21 EST by Gail Walter on behalf of School of Biological Sciences