Inhibition of Legionella Pneumophila by Bacillus sp.

Temmerman, R., Vervaeren, H., Noseda, B., Boon, N. and Verstraete, W. (2007) Inhibition of Legionella Pneumophila by Bacillus sp.. Engineering in Life Sciences, 7 5: 497-503. doi:10.1002/elsc.200620212

Author Temmerman, R.
Vervaeren, H.
Noseda, B.
Boon, N.
Verstraete, W.
Title Inhibition of Legionella Pneumophila by Bacillus sp.
Formatted title
Inhibition of Legionella pneumophila by Bacillus sp.
Journal name Engineering in Life Sciences   Check publisher's open access policy
ISSN 1618-2863
Publication date 2007-10-01
Sub-type Article (original research)
DOI 10.1002/elsc.200620212
Open Access Status
Volume 7
Issue 5
Start page 497
End page 503
Total pages 7
Place of publication Weinheim, Germany
Publisher Wiley-VCH Verlag GmbH
Language eng
Subject 0605 Microbiology
Abstract This study presents the potential of a biological control of Legionella pneumophila. It was verified to what extent the enrichment of various Bacillus species in water may decrease or prevent L. pneumophila from growing in water. During in vitro tests, B. subtilis BS104 was able to induce an average decrease in L. pneumophila numbers of 1.9 + 0.2 log units after 120 h. Furthermore, the spore and cell free filtrate of B. subtilis BS104 also decreased L. pneumophila by 2.6 + 0.4 log units after 120 h. An addition of Bacillus BS104 to a cooling tower test system with a water volume of 8 m3 resulted in a L. pneumophila level below 1000 CFU/L after 3 weeks, whereas the starting point was 5.3 × 104 CFU/L. Further experiments also indicated that B. subtilis BS104 might inhibit the internal replication of L. pneumophila in Acanthamoeba castellanii. This paper demonstrates that certain strains of Bacillus have the potential of reducing the number of viable L. pneumophila in water, or at least prevent its increase. These results are the first indication that a biological abatement of L. pneumophila could be possible.
Keyword Biological control
Health care
Pathogenic microorganisms
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Published Online: 10 Oct 2007

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
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Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 7 times in Scopus Article | Citations
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