Nuclear Pore Complex Structure and Plasticity Revealed by Electron and Atomic Force Microscopy

Maco, Bohumil, Fahrenkrog, Birthe, Huang, Ning-Ping and Aebi, Ueli (2006). Nuclear Pore Complex Structure and Plasticity Revealed by Electron and Atomic Force Microscopy. In X. Johné Liu (Ed.), Xenopus Protocols: Cell Biology and Signal Transduction Volume 322 ed. (pp. 273-287) Totowa, N.J.: Humana Press. doi:10.1007/978-1-59745-000-3_19


Author Maco, Bohumil
Fahrenkrog, Birthe
Huang, Ning-Ping
Aebi, Ueli
Title of chapter Nuclear Pore Complex Structure and Plasticity Revealed by Electron and Atomic Force Microscopy
Title of book Xenopus Protocols: Cell Biology and Signal Transduction
Place of Publication Totowa, N.J.
Publisher Humana Press
Publication Year 2006
Sub-type Other
DOI 10.1007/978-1-59745-000-3_19
Open Access Status Not yet assessed
Series Methods in Molecular Biology
Edition Volume 322
ISBN 978-1-58829-362-6 (Print) 978-1-59745-000-3 (Online)
Editor X. Johné Liu
Volume number 322
Chapter number 19
Start page 273
End page 287
Total pages 15
Total chapters 33
Language eng
Subjects 060112 Structural Biology (incl. Macromolecular Modelling)
Abstract/Summary To study the ultrastructure of nuclear pore complexes (NPCs), a wide spectrum of different electron microscopy (EM) or atomic force microscopy (AFM) techniques can be employed. The combination of these methods can reveal new insights into the structural and functional organization of this important supramolecular machine through which nucleocytoplasmic transport occurs. Negative staining, quick freezing/freeze-drying/rotary metal shadowing, embedding and thin sectioning, cryoelectron microscopy and tomography, scanning electron microscopy, or combination with immunolabeling techniques are tools for collecting data and information about the three-dimensional structure and architecture of the NPCs. AFM enables investigation of the functional dynamics of native NPCs under physiological conditions
Keyword Atomic force microscopy
electron microscopy
nuclear pore complex
nucleus
Xenopus laevis
Q-Index Code BX
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes ISSN: 1064-3745

 
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