Evaluation of the membrane-binding properties of the proximal region of the angiotensin II receptor (AT1A) carboxyl terminus by surface plasmon resonance

Kamimori, Hiroshi, Unabia, Sharon, Thomas, Walter G. and Aguilar, Marie-Isabel (2005) Evaluation of the membrane-binding properties of the proximal region of the angiotensin II receptor (AT1A) carboxyl terminus by surface plasmon resonance. Analytical Sciences, 21 2: 171-174. doi:10.2116/analsci.21.171


Author Kamimori, Hiroshi
Unabia, Sharon
Thomas, Walter G.
Aguilar, Marie-Isabel
Title Evaluation of the membrane-binding properties of the proximal region of the angiotensin II receptor (AT1A) carboxyl terminus by surface plasmon resonance
Journal name Analytical Sciences   Check publisher's open access policy
ISSN 0910-6340
1348-2246
Publication date 2005-01-01
Year available 2005
Sub-type Article (original research)
DOI 10.2116/analsci.21.171
Open Access Status DOI
Volume 21
Issue 2
Start page 171
End page 174
Total pages 4
Place of publication Tokyo, Japan
Publisher Nihon Bunseki Kagakkai
Language eng
Subject 060108 Protein Trafficking
060110 Receptors and Membrane Biology
060111 Signal Transduction
060199 Biochemistry and Cell Biology not elsewhere classified
060602 Animal Physiology - Cell
110201 Cardiology (incl. Cardiovascular Diseases)
110903 Central Nervous System
Abstract The proximal region of the angiotensin II receptor (AT1A) carboxyl-terminus (known as helix VIII) is important for receptor function. In this study, we used surface plasmon resonance (SPR) to examine the interaction of helix VIII-derived peptides with three model lipid membranes. The membrane-binding properties of these synthetic peptides, as well as a series of peptide analogues with modified amino acid sequences, could be explained by both amino acid sequence and kinetic binding data by SPR. The helix VIII peptides showed a higher affinity for lipid membranes that contained negatively charged phospholipid, rather than zwitterionic phospholipid. The findings of an SPR study may be useful for estimating the cooperative binding of intracellular receptor domains with G proteins and the components of the lipid bilayer.
Keyword Chemistry, Analytical
Chemistry
CHEMISTRY, ANALYTICAL
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
ERA 2012 Admin Only
School of Biomedical Sciences Publications
 
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Created: Thu, 15 Jan 2009, 19:07:52 EST by Ms Julie Anne Hansen on behalf of School of Biomedical Sciences