Evaluation of three methods for enriching H2-producing cultures from anaerobic sludge

Mu, Yang, Yu, Han-Qing and Wang, Gang (2007) Evaluation of three methods for enriching H2-producing cultures from anaerobic sludge. Enzyme and Microbial Technology, 40 4: 947-953. doi:10.1016/j.enzmictec.2006.07.033


Author Mu, Yang
Yu, Han-Qing
Wang, Gang
Title Evaluation of three methods for enriching H2-producing cultures from anaerobic sludge
Formatted title
Evaluation of three methods for enriching H2-producing cultures from anaerobic sludge
Journal name Enzyme and Microbial Technology   Check publisher's open access policy
ISSN 0141-0229
1879-0909
Publication date 2007-03-05
Year available 2006
Sub-type Article (original research)
DOI 10.1016/j.enzmictec.2006.07.033
Volume 40
Issue 4
Start page 947
End page 953
Total pages 7
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Formatted abstract
Hydrogen can be harvested from the microbial fermentation of organic substrates when methanogenesis is suppressed in an anaerobic digestion system. In this study three methods, heat-, acid- and alkaline-treatment, were used to suppress methanogenesis in mixed cultures and to enrich H2-producing inoculum. Highest H2 yield of 2.00 mol-H2/mol-glucose was achieved with the heat-treated sludge, while lowest yield of 0.48 mol-H2/mol-glucose was obtained with the alkaline-treated sludge. A butyrate-type fermentation was found for both heat- and alkaline-treated sludge, while a mixed-type fermentation occurred for the acid-treated sludge. A model was established to describe the kinetics of H2 production process and the yield coefficients of various products were estimated for the three cases with this model. The relationships among NADH/NAD+, oxidation–reduction potential and the H2 partial pressure were established and the evolvement of NADH/NAD+ and oxidation–reduction potential in the fermentative process for the three cases was also evaluated. The comparative experimental results show that the heat-treatment method was better than the two others for enriching H2-producing inoculums from mixed anaerobic cultures.
Keyword Acid-treated
Alkaline-treated
Anaerobic sludge
Heat-treated
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Available online 28 July 2006

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
 
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