Genome survey sequencing of the wine spoilage yeast Dekkera (Brettanomyces) bruxellensis

Woolfit, Megan, Rozpedowska, Elzbieta, Piskur,Jure and Wolfe, Kenneth H. (2007) Genome survey sequencing of the wine spoilage yeast Dekkera (Brettanomyces) bruxellensis. Eukaryotic cell, 6 4: 721-733. doi:10.1128/EC.00338-06


Author Woolfit, Megan
Rozpedowska, Elzbieta
Piskur,Jure
Wolfe, Kenneth H.
Title Genome survey sequencing of the wine spoilage yeast Dekkera (Brettanomyces) bruxellensis
Formatted title
Genome Survey Sequencing of the Wine Spoilage Yeast Dekkera (Brettanomyces) bruxellensis
Journal name Eukaryotic cell   Check publisher's open access policy
ISSN 1535-9778
1535-9786
Publication date 2007-04-01
Sub-type Article (original research)
DOI 10.1128/EC.00338-06
Open Access Status Not Open Access
Volume 6
Issue 4
Start page 721
End page 733
Total pages 13
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
The hemiascomycete yeast Dekkera bruxellensis, also known as Brettanomyces bruxellensis, is a major cause of wine spoilage worldwide. Wines infected with D. bruxellensis develop distinctive, unpleasant aromas due to volatile phenols produced by this species, which is highly ethanol tolerant and facultatively anaerobic. Despite its importance, however, D. bruxellensis has been poorly genetically characterized until now. We performed genome survey sequencing of a wine strain of D. bruxellensis to obtain 0.4x coverage of the genome. We identified approximately 3,000 genes, whose products averaged 49% amino acid identity to their Saccharomyces cerevisiae orthologs, with similar intron contents. Maximum likelihood phylogenetic analyses suggest that the relationship between D. bruxellensis, S. cerevisiae, and Candida albicans is close to a trichotomy. The estimated rate of chromosomal rearrangement in D. bruxellensis is slower than that calculated for C. albicans, while its rate of amino acid evolution is somewhat higher. The proteome of D. bruxellensis is enriched for transporters and genes involved in nitrogen and lipid metabolism, among other functions, which may reflect adaptations to its low-nutrient, high-ethanol niche. We also identified an adenyl deaminase gene that has high similarity to a gene in bacteria of the Burkholderia cepacia species complex and appears to be the result of horizontal gene transfer. These data provide a resource for further analyses of the population genetics and evolution of D. bruxellensis and of the genetic bases of its physiological capabilities.
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biological Sciences Publications
 
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Created: Sat, 20 Dec 2008, 01:52:25 EST by Diana Guillemin on behalf of School of Biological Sciences