Cystine uptake prevents production of hydrogen peroxide by Lactobacillus fermentum BR11.

Hung, J., Cooper, D., Turner, M. S., Walsh, T. and Giffard, P. M. (2003) Cystine uptake prevents production of hydrogen peroxide by Lactobacillus fermentum BR11.. Fems Microbiology Letters, 227 1: 93-99. doi:10.1016/S0378-1097(03)00653-0


Author Hung, J.
Cooper, D.
Turner, M. S.
Walsh, T.
Giffard, P. M.
Title Cystine uptake prevents production of hydrogen peroxide by Lactobacillus fermentum BR11.
Journal name Fems Microbiology Letters   Check publisher's open access policy
ISSN 0378-1097
1574-6968
Publication date 2003-01-01
Sub-type Article (original research)
DOI 10.1016/S0378-1097(03)00653-0
Volume 227
Issue 1
Start page 93
End page 99
Total pages 7
Place of publication Oxford
Publisher Blackwell
Language eng
Subject 0605 Microbiology
Abstract BspA is an abundant surface protein from Lactobacillus fermentum BR11, and is required for normal cystine uptake. In previous studies, a mutant strain deficient in BspA (L. fermentum PNG201) was found to be sensitive to oxidative stress. In this study, the biochemical basis for this was explored. It was found that under aerobic batch culture conditions in de Mann–Rogosa–Sharpe medium, both L. fermentum BR11 and PNG201 entered stationary phase due to hydrogen peroxide accumulation. However, this took place at a lower optical density for PNG201 than for BR11. Measurements of hydrogen peroxide levels revealed that the BspA mutant strain overproduces this compound. Addition of 6 mM cystine to aerobic cultures was found to prevent hydrogen peroxide production by both the BR11 and PNG201 strains, but lower cystine concentrations depressed hydrogen peroxide production in BR11 more efficiently than in PNG201. Each mole of cystine was able to prevent the production of several moles of hydrogen peroxide by L. fermentum BR11, suggesting that hydrogen peroxide breakdown is dependent upon a thiol that cycles between reduced and oxidized states. It was concluded that peroxide breakdown by L. fermentum BR11 is dependent upon exogenous cystine. It is most probable that the imported l-cystine is catabolized by a cystathionine lyase and then converted into a thiol reductant for a peroxidase.
Keyword Oxidative stress
Thiol
Peroxidase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Agriculture and Food Sciences
 
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Created: Sat, 13 Dec 2008, 08:13:18 EST by Dr Mark Turner on behalf of School of Land, Crop and Food Sciences