Antimicrobial activity of lysostaphin and a Listeria monocytogenes bacteriophage endolysin produced and secreted by lactic acid bacteria.

Turner, M. S., Waldherr F., Loessner M. J. and Giffard P. M. (2007) Antimicrobial activity of lysostaphin and a Listeria monocytogenes bacteriophage endolysin produced and secreted by lactic acid bacteria.. Systematic And Applied Microbiology, 30 1: 58-67. doi:10.1016/j.syapm.2006.01.013


Author Turner, M. S.
Waldherr F.
Loessner M. J.
Giffard P. M.
Title Antimicrobial activity of lysostaphin and a Listeria monocytogenes bacteriophage endolysin produced and secreted by lactic acid bacteria.
Journal name Systematic And Applied Microbiology   Check publisher's open access policy
ISSN 0723-2020
Publication date 2007-01-01
Sub-type Article (original research)
DOI 10.1016/j.syapm.2006.01.013
Volume 30
Issue 1
Start page 58
End page 67
Total pages 10
Place of publication Jena, Germany
Publisher Elsevier
Language eng
Subject 0605 Microbiology
Abstract The expression and secretion signals of the Sep protein from Lactobacillus fermentum BR11 were used to direct export of two peptidoglycan hydrolases by Lb. fermentum BR11, Lactobacillus rhamnosus GG, Lactobacillus plantarum ATCC 14917 and Lactococcus lactis MG1363. The production levels, hydrolytic and bacteriocidal activities of the Listeria monocytogenes bacteriophage N-acetylmuramoyl-l-alanine amidase endolysin Ply511 and the glycylglycine endopeptidase lysostaphin were examined. Buffering of the growth media to a neutral pH allowed detection of Ply511 and lysostaphin peptidoglycan hydrolytic activity from all lactic acid bacteria. It was found that purified Ply511 has a pH activity range similar to that of lysostaphin with both enzymes functioning optimally under alkaline conditions. Supernatants from lactobacilli expressing lysostaphin reduced viability of methicillin resistant Staphylococcus aureus (MRSA) by not, vert, similar8 log10 CFU/ml compared to controls. However, supernatants containing Ply511 were unable to control L. monocytogenes growth. In coculture experiments, both Lb. plantarum and Lb. fermentum synthesizing lysostaphin were able to effectively reduce MRSA cell numbers by >7.4 and 1.7 log10CFU/ml, respectively, while lactic acid bacteria secreting Ply511 were unable to significantly inhibit the growth of L. monocytogenes. Our results demonstrate that lysostaphin and Ply511 can be expressed in an active form from different lactic acid bacteria and lysostaphin showed superior killing activity. Lactobacilli producing lysostaphin may have potential for in situ biopreservation in foodstuffs or for prevention of S. aureus infections.
Keyword Lactobacillus
Lactococcus
Expression
Endolysin
Lysostaphin
Antimicrobial
Food
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Agriculture and Food Sciences
 
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Created: Sat, 13 Dec 2008, 07:57:32 EST by Dr Mark Turner on behalf of School of Land, Crop and Food Sciences