Activity of HIV entry and fusion inhibitors expressed by the human vaginal colonizing probiotic Lactobacillus reuteri RC-14

Liu, Janice J., Reid, Gregor, Jiang, Yonghou, Turner, Mark S. and Tsai, Che-Chung (2007) Activity of HIV entry and fusion inhibitors expressed by the human vaginal colonizing probiotic Lactobacillus reuteri RC-14. Cellular Microbiology, 9 1: 120-130. doi:10.1111/j.1462-5822.2006.00772.x


Author Liu, Janice J.
Reid, Gregor
Jiang, Yonghou
Turner, Mark S.
Tsai, Che-Chung
Title Activity of HIV entry and fusion inhibitors expressed by the human vaginal colonizing probiotic Lactobacillus reuteri RC-14
Journal name Cellular Microbiology   Check publisher's open access policy
ISSN 1462-5814
1462-5822
Publication date 2007-01-01
Sub-type Article (original research)
DOI 10.1111/j.1462-5822.2006.00772.x
Volume 9
Issue 1
Start page 120
End page 130
Total pages 11
Place of publication Oxford
Publisher Blackwell
Language eng
Subject 0605 Microbiology
Abstract Novel therapeutic approaches are needed to combat the rapid increase in HIV sexual transmission in women. The probiotic organism Lactobacillus reuteri RC-14 which safely colonizes the human vagina and prevents microbial infections, has been genetically modified to produce anti-HIV proteins which were capable of blocking the three main steps of HIV entry into human peripheral blood mononuclear cells. The HIV entry or fusion inhibitors were fused to the native expression and secretion signals of BspA, Mlp or Sep in L. reuteri RC-14 and the expression cassettes were stably inserted into the chromosome. L. reuteri RC-14 expressed the HIV inhibitors in cell wall-associated and secreted forms. L. reuteri RC-14 expressing CD4D1D2-antibody-like fusion proteins were able to bind single or dual tropic coreceptor-using HIV-1 primary isolates. This is the first study to show that a well-documented and proven human vaginal probiotic strain can express potent functional viral inhibitors, which may potentially lower the sexual transmission of HIV.
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Agriculture and Food Sciences
 
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Created: Sat, 13 Dec 2008, 07:55:04 EST by Dr Mark Turner on behalf of School of Land, Crop and Food Sciences