Determination of Haloperidol and Reduced Haloperidol in the Plasma and Blood of Patients on Depot Haloperidol

Eyles, Darryl W., Whiteford, Harvey A., Stedman, Terry J. and Pond, Susan M. (1992) Determination of Haloperidol and Reduced Haloperidol in the Plasma and Blood of Patients on Depot Haloperidol. Psychopharmacology, 106 2: 268-274. doi:10.1007/BF02801983


Author Eyles, Darryl W.
Whiteford, Harvey A.
Stedman, Terry J.
Pond, Susan M.
Title Determination of Haloperidol and Reduced Haloperidol in the Plasma and Blood of Patients on Depot Haloperidol
Journal name Psychopharmacology   Check publisher's open access policy
ISSN 0033-3158
Publication date 1992-06-01
Sub-type Article (original research)
DOI 10.1007/BF02801983
Volume 106
Issue 2
Start page 268
End page 274
Total pages 7
Place of publication Berlin
Publisher Springer
Language eng
Subject 06 Biological Sciences
Abstract We developed a sensitive HPLC assay to measure haloperidol (HA) and its metabolite, reduced haloperidol (RH), in plasma and whole blood. The conditions under which HA might be converted to RH during collection and analysis of blood were examined. Provided the blood was kept at 0° C, erythrocyte ketone reductase activity was insignificant. The solid phase extraction method did not generate RH. We studied ten patients taking 25–400 mg/month of HA decanoate and one patient for 4 weeks after the daily oral dose of 120 mg HA was ceased. In the patients on depot HA, the plasma and blood concentrations of HA were not significantly different (P>0.1). For the first time, RH was detected in plasma patients on depot drug, but only in three cases. In contrast, RH was present in the blood of eight of these patients. The accumulation of RH in red blood cells was also evident in the patient on oral HA, in whom the mean ratio of RH concentrations in whole blood to plasma was 3.6±1.1. Plasma concentrations of HA correlated highly with total neuroleptic activity measured by a radioreceptor assay. Compared to plasma, analysis of concentrations of HA and RH in blood has the advantages of greater sensitivity, of using smaller volumes of blood and of avoiding the efflux of HA and RH during separation of plasma and red cells.
Keyword Haloperidol
Reduced haloperidol
Depot haloperidol
HPLC
Plasma
Blood
Analysis
Ketone reductases
Pharmacokinetics
Metabolism
Therapeutic drug monitoring
Schizophrenia
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Faculty of Health and Behavioural Sciences -- Publications
School of Biological Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 21 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 29 Aug 2008, 02:20:57 EST