Role of calcium and vesicle-docking proteins in remobilising dormant neuromuscular junctions in desert frogs

Lavidis, N. A., Hudson, N. J., Choy, P. T., Lehnert, S. A. and Franklin, C. E. (2008) Role of calcium and vesicle-docking proteins in remobilising dormant neuromuscular junctions in desert frogs. Journal of Comparative Physiology A: Neuroethology Sensory Neural and Behavioral Physiology, 194 1: 27-37. doi:10.1007/s00359-007-0284-0


Author Lavidis, N. A.
Hudson, N. J.
Choy, P. T.
Lehnert, S. A.
Franklin, C. E.
Title Role of calcium and vesicle-docking proteins in remobilising dormant neuromuscular junctions in desert frogs
Journal name Journal of Comparative Physiology A: Neuroethology Sensory Neural and Behavioral Physiology   Check publisher's open access policy
ISSN 0340-7594
Publication date 2008-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1007/s00359-007-0284-0
Open Access Status Not yet assessed
Volume 194
Issue 1
Start page 27
End page 37
Total pages 10
Editor Fredrich G. Barth
Place of publication Germany
Publisher Springer
Language eng
Subject 970106 Expanding Knowledge in the Biological Sciences
0606 Physiology
0608 Zoology
Abstract Despite prolonged immobility the desert frog, Cyclorana alboguttata, suffers little impairment in muscle function. To determine compensatory mechanisms at neuromuscular junctions, transmitter release was examined along primary terminals in C. alboguttata iliofibularis muscle. Using extracellular recording we found the amplitudes of evoked endplate currents were significantly smaller in dormant frogs. In active frogs we identified two negatively sloping proximal–distal gradients of transmitter frequency and quantal content; a shallow proximal–distal gradient with low probability of transmitter release (<0.2) and a second much steeper proximal–distal gradient for quantal content with high probability release sites (>0.6). During aestivation, only a shallow gradient was identified. The high probability release sites in control frogs were inhibited during aestivation by a mechanism that could be reversed by (1) increasing the extracellular calcium concentration, and (2) increasing the frequency of stimulation. This suggests that transmitter vesicles are available during aestivation but not released. We quantified expression of messenger RNA transcripts coding for the transmitter vesicle-docking proteins synaptotagmin 1, syntaxin 1B and UNC-13. All three were rare transcripts maintained at control values during aestivation. Neuromuscular remobilisation after dormancy in C. alboguttata is more likely a product of rapidly reversible physiologic mechanisms than reorganisations of the neuromuscular transcriptome.
Keyword Aestivation
Neuromuscular Junction
Remobilisation
Calcium
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Biomedical Sciences Publications
Ecology Centre Publications
 
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Created: Wed, 30 Jul 2008, 02:19:40 EST