Targeting neural circuitry in zebrafish using GAL4 enhancer trapping

Scott, Ethan K., Mason, Lindsay, Arrenberg, Aristides B., Ziv, Limor, Gosse, Nathan J., Xiao, Tong, Chi, Neil C., Asakawa, Kazuhide, Kawakami, Koichi and Baier, Herwig (2007) Targeting neural circuitry in zebrafish using GAL4 enhancer trapping. Nature Methods, 4 4: 323-326. doi:10.1038/NMETH1033


Author Scott, Ethan K.
Mason, Lindsay
Arrenberg, Aristides B.
Ziv, Limor
Gosse, Nathan J.
Xiao, Tong
Chi, Neil C.
Asakawa, Kazuhide
Kawakami, Koichi
Baier, Herwig
Title Targeting neural circuitry in zebrafish using GAL4 enhancer trapping
Journal name Nature Methods   Check publisher's open access policy
ISSN 1548-7091
1548-7105
Publication date 2007-04-01
Sub-type Article (original research)
DOI 10.1038/NMETH1033
Volume 4
Issue 4
Start page 323
End page 326
Total pages 4
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Abstract We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
 
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Created: Fri, 18 Jul 2008, 21:36:25 EST by Laura McTaggart on behalf of School of Biomedical Sciences