Transient and stable transformation in mango by particle bombardment

Cruz-Hernandez, A., Town, L. J., Cavallaro, A. S. and Botella, J. R. (2000). Transient and stable transformation in mango by particle bombardment. In: S. Subhadrabandhu and A. Pichakum, Acta Horticulturae: Proceedings of the Sixth International Symposium on Mango. Sixth International Symposium on Mango, Pattaya City, Thailand, (237-242). 6-9 April 1999.


Author Cruz-Hernandez, A.
Town, L. J.
Cavallaro, A. S.
Botella, J. R.
Title of paper Transient and stable transformation in mango by particle bombardment
Conference name Sixth International Symposium on Mango
Conference location Pattaya City, Thailand
Conference dates 6-9 April 1999
Convener S. Subhadrabandhu
Proceedings title Acta Horticulturae: Proceedings of the Sixth International Symposium on Mango   Check publisher's open access policy
Journal name Proceedings of the Sixth International Symposium On Mango, Vols 1 and 2   Check publisher's open access policy
Place of Publication Leuven, Belgium
Publisher International Society for Horticultural Science
Publication Year 2000
Sub-type Fully published paper
ISBN 978-90-66058-62-0
ISSN 0567-7527
Editor S. Subhadrabandhu
A. Pichakum
Volume 509
Issue 509
Start page 237
End page 242
Total pages 6
Language eng
Formatted Abstract/Summary
 Genetic manipulation of plants requires a system for the introduction of foreign DNA into the cells. Particle Bombardment offers a rapid and efficient method for the delivery of DNA for transient and stable transformation studies. In this work we describe a system using mango proembryogenic masses of two polyembryonic cultivars (‘Carabao’ and ‘Kensington Pride’) as a target tissue for particle bombardment. The -glucuronidase (gus A) was used as reporter gene. The parameters associated with particle bombardment were optimized using transient expression assays of the -glucuronidase gene in proembryogenic masses. Osmotic treatment and particle acceleration pressure had a major effect on GUS transitory expression. Under optimal conditions, more than 1000 GUS foci were observed per microgram of bombarded DNA.
Subjects E2
270400 Botany
620205 Tropical fruit
Keyword Mangifera indica
genetic transformation
particle inflow gun
somatic embryogenesis
tropical crops
Q-Index Code E2

Document type: Conference Paper
Collection: School of Biological Sciences Publications
 
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Created: Fri, 06 Jun 2008, 23:28:52 EST