Monitoring human blood dendritic cell numbers in normal individuals and in stem cell transplantation

Fearnley, D. B., Whyte, L. F., Carnoutsos, S. A., Cook, A. H. and Hart, D. N. J. (1999) Monitoring human blood dendritic cell numbers in normal individuals and in stem cell transplantation. Blood, 93 2: 728-736.

Author Fearnley, D. B.
Whyte, L. F.
Carnoutsos, S. A.
Cook, A. H.
Hart, D. N. J.
Title Monitoring human blood dendritic cell numbers in normal individuals and in stem cell transplantation
Journal name Blood   Check publisher's open access policy
ISSN 0006-4971
Publication date 1999-01-01
Sub-type Article (original research)
Volume 93
Issue 2
Start page 728
End page 736
Total pages 9
Place of publication Philadelphia, PA, USA
Publisher W B Saunders Co
Language eng
Subject C1
320300 Medical Biochemistry and Clinical Chemistry
730108 Cancer and related disorders
Abstract Dendritic cells (DC) originate from a bone marrow (BM) precursor and circulate via the blood to most: body tissues where they fulfill a role in antigen surveillance. Little is known about DC numbers in disease, although the reported increase in tissue DC turnover due to inflammatory stimuli suggests that blood DC numbers may be altered in some clinical situations. The lack of a defined method for counting De has limited patient studies. We therefore developed a method suitable for routine monitoring of blood DC numbers, using the CMRF44 monoclonal antibody (MoAb) and flow cytometry to identify DC. A normal range was determined from samples drawn from 103 healthy adults. The mean percentage of DC present in blood mononuclear cells (MNC) was 0.42%, and the mean absolute DG count was 10 x 10(6) DC/L blood. The normal ranges for DC (mean +/- 1.96 standard deviation [SD]) were 0.15% to 0.70% MNC or 3 to 17 x 106 DC/L blood. This method has applications for monitoring attempts to mobilize DC into the blood to facilitate their collection for immunotherapeutic purposes and for counting blood DC in other patients. In preliminary studies, we have found a statistically significant decrease in the blood DC counts in individuals at the time of blood stem cell harvest and in patients with acute illnesses, including allogeneic bone marrow transplant (BMT) recipients with acute graft-versus-host disease (aGVHD). (C) 1999 by The American Society of Hematology.
Keyword Hematology
Colony-stimulating Factor
Human Bone-marrow
Tumor-necrosis-factor
Peripheral-blood
Langerhans Cells
Hematopoietic Progenitors
Allostimulatory Cells
Inflammatory Response
Monoclonal-antibody
Flow-cytometry
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Wed, 11 Jun 2008, 01:52:35 EST