Physical mapping of ten equine dinucleotide repeat microsatellites

Lear, T. L., Brandon, Richard Bruce and Bell, Thomas Kevin (1999) Physical mapping of ten equine dinucleotide repeat microsatellites. Animal Genetics, 30 3: 235-235. doi:10.1046/j.1365-2052.1999.00404-15.x


Author Lear, T. L.
Brandon, Richard Bruce
Bell, Thomas Kevin
Title Physical mapping of ten equine dinucleotide repeat microsatellites
Journal name Animal Genetics   Check publisher's open access policy
ISSN 0268-9146
1365-2052
0268-9154
Publication date 1999-06-01
Sub-type Article (original research)
DOI 10.1046/j.1365-2052.1999.00404-15.x
Open Access Status Not Open Access
Volume 30
Issue 3
Start page 235
End page 235
Total pages 1
Place of publication Oxford, U.K.
Publisher Blackwell Scientific Publications
Language eng
Subject C4
270200 Genetics
630107 Minor livestock (e.g. horses, goats, deer)
Abstract Twenty equine microsatellites were isolated from a genomic phage library, and their genetical and physical localization was sought by linkage mapping and fluorescent in situ hybridization (FISH). Nineteen of the markers were found to be polymorphic with, in most cases, heterozygosities exceeding 50%. The markers were mapped in a Swedish reference family for gene mapping, comprising eight half-sib families from Standardbred and Icelandic horse sires. Segregation was analyzed against a set of 35 other markers typed in the pedigree. Thirteen of the microsatellites showed linkage to at least one other marker, with a total of 21 markers being involved in these linkages. In parallel, 18 of the microsatellites could be assigned to their chromosomal region by FISH. These assignments involved eight equine autosomes: ECA1, 2, 4, 6, 9, 10, 15, and 16. The genetical and physical mappings revealed by this study represent a significant extension of the current knowledge of the equine genome map.
Formatted abstract
Nine clones mapped to single sites. ASB21 mapped to numerous sites an may contain repetitive elements common to the centromeres of several chromosomes. Linkage data supports the assignments of ASB23 and ASB25. The clone containing ASB38 may be chimeric as the microsat ellite marker maps to ECA27 by linkage analysis
© 1999 International Society for Animal Genetics
Q-Index Code C4
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: QAAFI Biological Information Technology (QBIT) Publications
 
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Created: Wed, 11 Jun 2008, 01:19:58 EST