Characterization of ATDRG1, a member of a new class of GTP-binding proteins in plants

Etheridge, N, Trusov, Y, Verbelen, JP and Botella, JR (1999) Characterization of ATDRG1, a member of a new class of GTP-binding proteins in plants. Plant Molecular Biology, 39 6: 1113-1126. doi:10.1023/A:1006137221259


Author Etheridge, N
Trusov, Y
Verbelen, JP
Botella, JR
Title Characterization of ATDRG1, a member of a new class of GTP-binding proteins in plants
Journal name Plant Molecular Biology   Check publisher's open access policy
ISSN 0167-4412
Publication date 1999-04-01
Year available 1999
Sub-type Article (original research)
DOI 10.1023/A:1006137221259
Open Access Status Not yet assessed
Volume 39
Issue 6
Start page 1113
End page 1126
Total pages 14
Place of publication Dordrecht, Netherlands
Publisher Kluwer Academic Publishers
Language eng
Subject C1
270800 Biotechnology
780105 Biological sciences
Abstract We report the initial characterization of an Arabidopsis thaliana cDNA (atdrg1), a member of a new class of GTP-binding proteins (G-proteins) in plants. The predicted ATDRG1 protein contains all five structural motifs characteristic of the G-protein superfamily. Apart from these motifs, the amino acid sequence differs substantially from all known G-proteins except for a recently discovered new family named developmentally regulated G-proteins (DRGs). Sequences closely related to atdrg1 are found in species as distant as human (80% amino acid conservation), Drosophila (74%), yeast (77%) and Caenorhabditis elegans (77%). The remarkable evolutionary conservation of these proteins suggests an important, but as yet unclear role. Phylogenetic analysis of the available homologous sequences strongly suggests a diphyletic origin of the eukaryotic DRG proteins. Northern analysis shows high levels of atdrg1 mRNA in all Arabidopsis tissues studied, and homologues of atdrg1 are present throughout the plant kingdom. In situ hybridization reveals that atdrg1 is highly expressed in actively growing tissues and reproductive organs. Southern analysis indicates the presence of either one or two copies of atdrg1 in the Arabidopsis genome. Immunolocalization studies show that the protein is present in cytoplasmic vesicles found mainly in actively growing tissues suggesting a putative role for ATDRG1 in either the regulation of vesicle transport or the regulation of enzymes involved in storage protein processing.
Keyword Biochemistry & Molecular Biology
Plant Sciences
Arabidopsis
Gtp-binding Proteins
Immunolocalization
Signal Transduction
Phylogenetic Trees
Expression
Sequence
Drg
Identification
Genes
Family
Wheat
Ras
Rna
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Biological Sciences Publications
School of Chemistry and Molecular Biosciences
 
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Created: Wed, 11 Jun 2008, 01:13:04 EST