Zymographic analysis of equine laminitis

Mungall, BA and Pollitt, CC (1999) Zymographic analysis of equine laminitis. Histochemistry and Cell Biology, 112 6: 467-472. doi:10.1007/s004180050430


Author Mungall, BA
Pollitt, CC
Title Zymographic analysis of equine laminitis
Journal name Histochemistry and Cell Biology   Check publisher's open access policy
ISSN 0948-6143
Publication date 1999-01-01
Sub-type Article (original research)
DOI 10.1007/s004180050430
Volume 112
Issue 6
Start page 467
End page 472
Total pages 6
Place of publication Germany
Publisher Springer-Verlag
Language eng
Subject C1
780105 Biological sciences
300500 Veterinary Sciences
Abstract To investigate the role of matrix metalloproteinase (MMP) activity in the pathophysiology of equine laminitis, the techniques of in situ zymography and quantitative SDS-PAGE zymography were used to analyse the lamellae and plasma and serum of horses with carbohydrate overload-induced laminitis. The gelatinase activity localised within the epidermal lamellae of laminitic hooves did not differ significantly from normal hooves. In laminitis sections there was an increase in vascular gelatinase activity, possibly associated with the perivascular cuffing of polymorphonucleocytes. Both plasma and serum samples from horses developing laminitis showed a rapid increase in the concentration of circulating latent MMP-9, while MMP-2 remained relatively constant. These results support the hypothesis that laminitis histopathology results from an inadequate regulation of gelatinase activity, resulting in selective degradation of basement membrane components, leading to laminitis due to failure of the basement membrane-epidermis attachment.
Keyword Cell Biology
Microscopy
Matrix Metalloproteinases
Basement-membrane
Iv Collagenase
Cleavage
Cells
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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Created: Wed, 11 Jun 2008, 00:55:21 EST