Changing patterns of spatial buffering of glutamate in developing rat retina are mediated by the Muller cell glutamate transporter GLAST

Pow, D. V. and Barnett, N. L. (1999) Changing patterns of spatial buffering of glutamate in developing rat retina are mediated by the Muller cell glutamate transporter GLAST. Cell andTissue Research, 297 1: 57-66. doi:10.1007/s004410051333


Author Pow, D. V.
Barnett, N. L.
Title Changing patterns of spatial buffering of glutamate in developing rat retina are mediated by the Muller cell glutamate transporter GLAST
Journal name Cell andTissue Research   Check publisher's open access policy
ISSN 0302-766X
1432-0878
Publication date 1999-06-01
Year available 1999
Sub-type Article (original research)
DOI 10.1007/s004410051333
Open Access Status DOI
Volume 297
Issue 1
Start page 57
End page 66
Total pages 10
Editor K. Unsicker
Brenda Russell
W. W. Franke
J. R. Sladek
A. Oksche
Place of publication New York
Publisher Springer Verlag
Language eng
Subject C1
270107 Cell Neurochemistry
730111 Hearing, vision, speech and their disorders
Abstract The patterns of expression of the glutamate transporter GLAST were compared with the patterns of uptake of exogenous D-aspartate, which is a substrate for all glutamate transporters. At postnatal day 0, fine radial processes and end feet of presumptive Müller cells were weakly immunoreactive for GLAST. At postnatal day 3, intense labelling was associated with astrocytes enveloping newly formed blood vessels on the vitread surface of the retina. Between postnatal days 7 and 10, there was a rapid increase in the intensity of labelling in the Müller cells but clear stratification of GLAST-immunoreactive processes in the inner plexiform layer was not observed until postnatal day 14. By comparison, D-aspartate uptake was initially associated with a wide variety of cellular elements including most neuroblasts, presumptive Müller cells, and astrocytes associated with blood vessels but was absent from the somata of many neurons in the ganglion cell layer and amacrine cell layer. There was a gradual contraction in the numbers of cells that were able to take up D-aspartate, such that, by adulthood, uptake was restricted mainly to Müller cells and astrocytes. We conclude that, during early retinal development, the low levels of GLAST expression by Müller cells permit D-aspartate, and by inference, glutamate, to permeate the retina freely, thus allowing uptake by other glutamate transporters on other cell types. As the retina matures, increased expression of GLAST by Müller cells restricts the access of D-aspartate to other cellular compartments in the retina. This changing pattern of spatial buffering of glutamate by GLAST probably has significant implications regarding our understanding of the role of glutamate during processes such as retinal synaptogenesis.
Keyword Key words D-aspartate
Development
Glutamate
Retina
Glutamate transporter (GLAST)
Rat (Wistar)
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Faculty of Health and Behavioural Sciences -- Publications
 
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Created: Wed, 11 Jun 2008, 00:40:19 EST