Expression of transforming growth factor-beta receptors types II and III within various cells in the rat periodontium

Gao, Jin, Symons, Anne L. and Bartold, P. Mark (1999) Expression of transforming growth factor-beta receptors types II and III within various cells in the rat periodontium. Journal of Peridontal Research, 34 2: 113-122. doi:10.1111/j.1600-0765.1999.tb02230.x


Author Gao, Jin
Symons, Anne L.
Bartold, P. Mark
Title Expression of transforming growth factor-beta receptors types II and III within various cells in the rat periodontium
Journal name Journal of Peridontal Research   Check publisher's open access policy
ISSN 0022-3484
Publication date 1999-02-01
Sub-type Article (original research)
DOI 10.1111/j.1600-0765.1999.tb02230.x
Volume 34
Issue 2
Start page 113
End page 122
Total pages 10
Place of publication Hoboken, NJ
Publisher Wiley
Language eng
Subject C1
730112 Oro-dental and disorders
1105 Dentistry
Formatted abstract
This study reports the immunohistochemical localization of TGF-β receptor type II (TβR-II) and type III (TβR-III) in cells of the forming periodontal ligament (PDL) in rat first molar roots.

Mandibular periodontium was obtained from 3, 6 and 12-wk-old rats. This represented tissue from the initial, premature and post-mature stages of root and periodontal development, respectively. Mandibular bone chips and molar roots were used to isolate osteoblasts, fibroblasts and cementoblasts. Cells were obtained using a 2-step trypsinization and explant technique, and cultured in Dulbecco's modification of Eagle's medium (DMEM) under routine cell culture conditions.
Cells were cultured on coverslips for the purpose of detecting TGF-β receptors, and compared with whole tissue sections using the same detection method. Cells which stained positively for TβR-II and TβR-III on both paraffin sections and cultured cell slides were counted.
Both receptors were expressed in the various periodontal tissue compartments. PDL fibroblasts, cementoblasts and osteoblasts were stained positively for TβR-II and TβR-III. Endothelial cells were noted to be positive for TβR-II only. TβR-II was more widely distributed in cells than TβR-III, but TβR-III was extensively localized in the extracellular matrix. Both receptors were expressed on the cell membrane and also localized in the cytoplasm.

The findings for paraffin sections were consistent with the immunohistochemical staining of cultured cells. The percentage of cells which stained positively for TβR-II was greater (∼85%) than that for TβR-III (∼60%) in all major types of the PDL cells on both paraffin sections and cultured cell slides. Extensive location of TGF-β receptors in both cells and extracellular matrix suggests that several binding sites are available for TGF-βs to interact with target cells during development and following maturation of the periodontium.
Keyword Cell Culture
Immunohistochemistry
Pdl Cells
Rats
Dentistry, Oral Surgery & Medicine
Tgf-b Receptor Types Ii And Iii
Tgf-beta Receptor Types Ii And Iii
TGF-β Receptor Types Ii And Iii
Signal-transduction
Proteoglycan Betaglycan
Invitro
System
Kinase
Purification
Complex
Ligands
Cloning
Forms
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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Created: Wed, 11 Jun 2008, 00:37:24 EST