Long-term maintenance of low concentrations of fructose for the study of hepatic glucose phosphorylation

Phillips, John W., Henly, Debra C. and Berry, Michael N. (1999) Long-term maintenance of low concentrations of fructose for the study of hepatic glucose phosphorylation. Biochemical Journal, 337 3: 497-501. doi:10.1042/0264-6021:3370497


Author Phillips, John W.
Henly, Debra C.
Berry, Michael N.
Title Long-term maintenance of low concentrations of fructose for the study of hepatic glucose phosphorylation
Journal name Biochemical Journal
ISSN 0006-2936
0264-6021
Publication date 1999-02-01
Sub-type Article (original research)
DOI 10.1042/0264-6021:3370497
Open Access Status Not Open Access
Volume 337
Issue 3
Start page 497
End page 501
Total pages 5
Place of publication London, U.K.
Publisher Portland Press
Language eng
Subject C1
321206 Preventive Medicine
730105 Endocrine organs and diseases (incl. diabetes)
0601 Biochemistry and Cell Biology
1101 Medical Biochemistry and Metabolomics
Formatted abstract
The stimulation of glucose phosphorylation in isolated hepatocytes by low fructose concentrations is transient due to the rapid metabolism of fructose. To prolong this stimulatory effect fructose was enzymically generated in the incubation medium from either sucrose with invertase or inulin with inulinase. A maximal rate of glucose phosphorylation was achieved when fructose was formed at at least 0.01 µmol/min, which maintained a concentration of 70 µM fructose in the medium. In the presence of a fructose concentration of 70 µM, the rate of phosphorylation with 5 mM glucose was doubled and remained constant over a 2.5 h period. Under these conditions the rate of glycolysis was increased more than 3-fold. The stimulation of flux through glucokinase by low concentrations of fructose decreased the proportion of glucose phosphorylated, which was cycled between glucose and glucose 6-phosphate, and increased the proportion that was glycolysed. The method described for maintaining the stimulation of glucose phosphorylation by isolated hepatocytes over prolonged incubation periods is especially suited to the further study of the control of glucokinase activity, in particular how the variation of flux through glucokinase affects the flux through all the pathways that utilize the product, glucose 6-phosphate.
© 1999 Biochemical Society
Keyword Biochemistry & Molecular Biology
Glucokinase
Hepatocyte
Inulinase
Isolated Rat Hepatocytes
Glucokinase Translocation
Carbohydrate-metabolism
Regulatory Protein
Liver
Gluconeogenesis
Stimulation
Glycolysis
6-phosphate
Lactate
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Wed, 11 Jun 2008, 00:08:38 EST