Properties of the bovine viral diarrhoea virus replicase in extracts of infected MDBK cells

Warrilow, D., Lott, W. B., Greive, S. and Gowans, Eric J. (2000) Properties of the bovine viral diarrhoea virus replicase in extracts of infected MDBK cells. Archives of Virology, 145 10: 1-9. doi:10.1007/s007050070046


Author Warrilow, D.
Lott, W. B.
Greive, S.
Gowans, Eric J.
Title Properties of the bovine viral diarrhoea virus replicase in extracts of infected MDBK cells
Journal name Archives of Virology   Check publisher's open access policy
ISSN 0304-8608
1432-8798
0939-1983
Publication date 2000-10-01
Sub-type Article (original research)
DOI 10.1007/s007050070046
Volume 145
Issue 10
Start page 1
End page 9
Total pages 9
Place of publication Veinna, Austria.
Publisher Springer-Verlang Wien
Language eng
Subject C1
Formatted abstract
An assay for the bovine viral diarrhoea virus (BVDV) replicase was developed using extracts from BVDV-infected cells. The replicase activity was maximal approximately 8 h post-infection as measured by the generation of a genomic length radiolabelled RNA. Using a semi-denaturing gel system, three virus-specific in vitro radiolabelled nascent RNA species were identified. A fast-migrating RNA was demonstrated to be the double-stranded replicative form (RF). A second form was shown to be a partially single-stranded/partially double-stranded RNA, characteristic of the replicative intermediate (RI). A third form, which was often undetectable, migrated between the RF and RI and was probably genomic viral RNA. The optimal replicase activity was dependent on 5–10 mM Mg2+ and although it was also active in 1–2 mM Mn2+ it was inhibited at higher concentrations. The optimum KCl concentration for labelling of the RI and RF were different, suggestive of at least two distinct replicase activities. These results are supportive of a semi-conservative model of BVDV RNA replication.
© Springer-Verlag 2000
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: Clinical Medical Virology Centre Publications
 
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Created: Tue, 10 Jun 2008, 23:23:01 EST