The human immunodeficiency virus type 1 TAR RNA upper stem-loop plays distinct roles in reverse transcription and RNA pagaging

Harrich, David, Hooker, C. William and Parry, Emma (2000) The human immunodeficiency virus type 1 TAR RNA upper stem-loop plays distinct roles in reverse transcription and RNA pagaging. Journal of Virology, 74 12: 5639-5646. doi:10.1128/JVI.74.12.5639-5646.2000

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Author Harrich, David
Hooker, C. William
Parry, Emma
Title The human immunodeficiency virus type 1 TAR RNA upper stem-loop plays distinct roles in reverse transcription and RNA pagaging
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
Publication date 2000-06-01
Year available 2000
Sub-type Article (original research)
DOI 10.1128/JVI.74.12.5639-5646.2000
Open Access Status File (Publisher version)
Volume 74
Issue 12
Start page 5639
End page 5646
Total pages 8
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Subject C1
270303 Virology
730101 Infectious diseases
Formatted abstract
The human immunodeficiency virus type 1 (HIV-1) RNA genome is flanked by a repeated sequence (R) that is required for HIV-1 replication. The first 57 nucleotides of R form a stable stem-loop structure called the transactivation response element (TAR) that can interact with the virally encoded transcription activator protein, Tat, to promote high levels of gene expression. Recently, we demonstrated that TAR is also important for efficient HIV-1 reverse transcription, since HIV-1 mutated in the upper stem-loop of TAR showed a reduced ability both to initiate and to complete reverse transcription. We have analyzed a series of HIV-1 mutant viruses to better defined the structural or sequence elements required for natural endogenous reverse transcription and packaging of virion RNA. Our results indicate that the requirement for TAR in reverse transcription is conformation dependent, since mutants with mutations that alter the upper stem-loop orientation are defective for reverse transcription initiation and have minor defects in RNA packaging. In contrast, TAR mutations that allowed the formation of alternative upper stem-loop structure greatly reduced RNA packaging but did not affect reverse transcription efficiency. These results are consistent with direct involvement of the upper stem-loop structure in packaging of genomic RNA and suggest that the TAR RNA stem-loop from nucleotide +18 to +42 interacts with other components of the reverse transcription initiation complex to promote efficient reverse transcription.
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Tue, 10 Jun 2008, 22:40:52 EST